FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412072

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412072
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1679287
Sequences flagged as poor quality0
Sequence length91
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTACAGAGCCCCGAATTAATACCAATAGAAGGGCAATGCTTTTAGAT30270.18025507253971476No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT28810.17156090650377215No Hit
GACTTAAACAGCTTAAAGTTTAGTTTAAAAGTTGTAGGTGATTAAAATAA25870.15405347626701094No Hit
CTGTAGTCCTTTCTCTCAATTTTCTTTTAAATACATTTTTTACTCCATGA25710.15310069094800352No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23620.1406549327184692No Hit
GTGCATATTGGCGGCGCACGCCTCATTACGATTCGCCTGCTTGCTTCTCC20740.12350479697633579No Hit
CTTCTAAGTCTTCATATTTTCTCTTAAAATCTTAAGCTATTAAAATTACG20430.12165877542075892No Hit
GTTTAAGTCACCTTCATTTTAATCTAAAAGCATTGCCCTTCTATTGGTAT20300.12088463734906542No Hit
CTTCTATCTTCTCCAAATTGTTTCATCCTACCACTCCCAATTAATCTTTC20080.11957455753543023No Hit
GGAATGTACAGTGCATATTGGCGGCGCACGCCTCATTACGATTCGCCTGC19830.11808583047448114No Hit
GTACATTCCACAAGCATTGCCTTCTTATTTTACTTCTTTTAGCTGTTTAA19450.11582296534183853No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19400.11552521992964872No Hit
AATATGCACTGTACATTCCACAAGCATTGCCTTCTTATTTTACTTCTTTT18500.11016580251023202No Hit
GGCTTCTGCTGAGGGGGCAGGCGGAGCTTGAGGAAACCGCAGATAAGTTT18380.10945121352097646No Hit
CAATTAATCTTTCCATTTTCGTCTGCGTTTAGTAAATGCGTTAACTAGGC17810.10605691582201256No Hit
GCGCAGGGGCTTCTGCTGAGGGGGCAGGCGGAGCTTGAGGAAACCGCAGA16980.1011143419796616No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC16300.051.6310122
GTGGTAT15950.049.0331951
CTAACGC452.9250094E-547.2284283
TCTAACG651.06025254E-745.773882
GGTATCA33850.034.028571
GTATCAA59650.033.062871
TATCAAC73500.031.1698342
CGCAGTA703.941659E-430.360232
CAACGCA77150.029.6960585
ATCAACG77150.029.5858693
TCAACGC77500.029.4522554
AACGCAG89600.025.9018426
ATACGGG3950.025.8254091
CGCAGAG91650.024.9021058
CCGGTGC1201.2765389E-524.791978
TAACGCC1051.4697087E-424.2889044
GCAGAGT98200.023.326299
AGTCGAG16050.023.3024657
ATTGCCG552.0176842E-423.18072322-23
ACGCAGA101400.022.8848957
Produced by FastQC (version 0.11.5)