FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412074

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412074
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1755157
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT41480.23633213439025683No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30570.1741724529486536No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27250.1552567662038211No Hit
ATATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA26210.14933137035604221No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT25730.14659657227245199No Hit
GACCAGACTTCGCTCGTACTCGTGCGCCTCGCTTCGCTTTTCCTCCGCAA24380.13890495266235442No Hit
AATATGCACTGTACATTCCACAAGCATTGCCTTCTTATTTTACTTCTTTT23620.13457485569666988No Hit
GTACATTCCACAAGCATTGCCTTCTTATTTTACTTCTTTTAGCTGTTTAA22760.12967500913023736No Hit
GTGCATATTGGCGGCGCACGCCTCATTACGATTCGCCTGCTTGCTTCTCC22580.128649459848891No Hit
GGAATGTACAGTGCATATTGGCGGCGCACGCCTCATTACGATTCGCCTGC21110.1202741407178959No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT18240.10392232717642921No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTAC150.001029050885.0124741
GTGGTAT20150.054.002961
TGGTATC22250.051.7686962
AAGTACG9850.047.0373571
GCGTTAT603.2065855E-642.5062371
GGTATCA42700.035.9361881
TCGTACG301.595889E-435.41782826-27
ATACGGG8100.034.6347121
GTATCAA75350.033.1700971
TATCAAC99900.031.6119482
AGTATCA9350.029.5497931
TCGAGGT20150.029.5269819
ATCAACG111000.028.4890463
GTCGAGG22500.028.1429658
CAACGCA112200.027.9949385
TCAACGC113200.027.822734
CGTTATA1106.494287E-627.0478862
AACGCAG120650.026.0694796
ATATCAA26500.025.9849471
TGCGTGA1950.025.06349854-55
Produced by FastQC (version 0.11.5)