FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412076

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412076
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1158677
Sequences flagged as poor quality0
Sequence length91
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT13210.11400933996273337No Hit
GACCAGACTTCGCTCGTACTCGTGCGCCTCGCTTCGCTTTTCCTCCGCAA12540.1082268829017923No Hit
GTGCATATTGGCGGCGCACGCCTCATTACGATTCGCCTGCTTGCTTCTCC11880.10253073117011903No Hit
GGAATGTACAGTGCATATTGGCGGCGCACGCCTCATTACGATTCGCCTGC11750.10140876188963792No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGT250.007790791351.0090686
GGTATCA16400.037.8453561
TACCCGC450.001663363137.776348
AAGTACG2850.035.7989271
TGGTATC10700.034.9579772
GTGCGTG250.002788908334.0016454-55
GTACTAG2300.031.421341
GTGGTAT11100.031.4046881
GTTAGCC703.9391557E-430.3625373
GTATCAA29600.027.0003741
CTAGGCA2850.026.8468764
TACTAGG2800.025.8070432
CCCCATA2159.094947E-1225.7011342
AGTCGAG9000.025.499037
TATCAAC37400.025.3443162
CAACGCA38400.024.574685
ATCAACG38600.024.447353
TACCGAT2101.72804E-1024.284797
TCAACGC38950.024.1185344
GCATCGT2302.0008883E-1124.0208248
Produced by FastQC (version 0.11.5)