FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412077

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412077
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1158677
Sequences flagged as poor quality0
Sequence length61
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG43860.3785351741684697No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGCCG351.5715323E-647.1446342
CGGTAAG401.9001693E-434.3792640
CGCTATG401.901134E-434.37629344
TGCGACG1003.6379788E-1232.99981722
ATGCGAC857.585186E-1032.35276421
GTCGTCT957.094059E-1131.84055753
CGTCTAC1001.3096724E-1030.24852655
TCGCCGG553.4899676E-530.00113143
GTGGTAT10100.029.41341
CAATGCG1052.3101165E-1028.80687719
GGCTCGC606.3124724E-527.5034140
CGATAGT400.00787005327.50222411
CTCGGTA507.0304045E-427.50103638
CGGCTAA904.7179128E-827.50103642
AGGTCGT1103.965397E-1027.4998522
GTACCGT606.318044E-527.49984732
TATGTCG507.0339546E-427.49866153
CTCGTTA400.00787502927.4986655
TAGCGTG1303.6379788E-1227.4974755
TGGTATC10650.027.3671992
Produced by FastQC (version 0.11.5)