FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412078

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412078
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1397392
Sequences flagged as poor quality0
Sequence length91
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT25630.18341310097667654No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22890.1638051455854907No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18300.1309582422112049No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTACG4200.053.6419261
GTGGTAT16350.049.3984871
TGGTATC18850.045.3227122
ATACGGG3350.041.874241
GGTATCA29300.036.9956251
GTATCAA50000.030.2661931
TATCAAC62850.029.6886482
TCGTAAC300.006807562428.33304844-45
TACCGCG600.006807604328.3320357
CAACGCA66550.027.5261085
ATCAACG67500.027.3905743
TCAACGC67350.027.2622554
AACGCAG68800.026.625916
GTCGAGG9350.026.3624278
GACCGTT1001.05640946E-425.4988298
AGTCGAG12950.023.628267
CGCAGAG78850.023.2297538
ACGCAGA80250.022.82457
ATATCAA17300.022.3600651
GCAGAGT83300.022.0398049
Produced by FastQC (version 0.11.5)