FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412079

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412079
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1397392
Sequences flagged as poor quality0
Sequence length61
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG72350.5177502089606925No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTCTA1000.038.50160654
TACGATC300.001925371536.6681942
GTGGTAT15500.033.7207371
TGGTATC15800.033.0712
CGTAAGC606.316934E-527.50114642
AGTCGTA507.030971E-427.5011469
GTCGTCT1303.6379788E-1227.50114453
TAGTACG507.039802E-427.495244
CGTCTAC1450.026.5528355
GTATCAA52950.024.9893111
TCGTCAG1551.8189894E-1224.83974541
GTCGTCA1551.8189894E-1224.83885424
GGTATCA27100.024.2607271
CGCGATA801.6084807E-524.06264114
AATACGA801.6084807E-524.06264124
CTAGTAC2300.022.7150863
AGGTCGT1757.2759576E-1222.00012822
AGCTCGT1658.367351E-1121.66834438
GGTCGTC2050.021.46277252
TATCAAC61900.021.2367482
Produced by FastQC (version 0.11.5)