FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412082

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412082
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1969104
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT50700.2574775126148746No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39530.20075120460879667No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30260.1536739552608699No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT28510.14478666439152021No Hit
ATATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA24930.12660580649879336No Hit
GTGTATAAGCATATCAATATTAAAAAGCAAGCAAGCAGAATTTGGAATTC23750.120613233226889No Hit
ATCTTGGGCTGTGACAAAGTCACATGGTTCACACGGCAGGCATACTCATC20810.10568258456638145No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT20490.1040574799502718No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACGGG4550.061.659661
GTGGTAT23600.055.4762761
TGGTATC25850.054.0953372
AAGTACG14350.037.620141
GGTATCA43300.034.9486731
GTATCAA79550.033.717691
TATCAAC104250.031.6788542
TCAACGC113250.029.2356454
ATCAACG113100.029.2000033
CAACGCA115050.028.7043575
GTCGAGG22550.028.0807728
TCGAGGT22400.027.3195029
AACGCAG123450.026.7856376
AGTCGAG28450.026.7393677
GTGCACG808.6044194E-426.5673731
ATAACGC808.6095254E-426.5646743
AGTATCA11050.026.5433311
CCTAGTA2900.026.381472
CTACGCG501.05669926E-425.49949852-53
CGATAGT501.0568816E-425.4988520-21
Produced by FastQC (version 0.11.5)