FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412083

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412083
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1969104
Sequences flagged as poor quality0
Sequence length61
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG105150.5339992199497843No Hit
GTGTATAAGCATATCAATATTAAAAAGCAAGCAAGCAGAATTTGGAATTCATCCAATCCAA23630.12000381899584785No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT26200.041.705291
TGGTATC28450.038.2820132
CGCGTAT553.4924255E-529.99958424
TCGCGTA606.320615E-527.49961923
GGTATCA52600.025.378031
TAGCGAC550.001226601225.00092312
CTACGCG550.001226601225.00092352
CCCATCG550.001226601225.00092339
CGACCAT550.001227691524.99711233
GTATCAA96350.024.8239021
AACGCTC901.4477937E-624.44472947
CGTCTAC1251.7462298E-924.20089355
CTGTGCG1900.023.159349
AGAGTAC63150.022.95017411
GCGACCA600.00203560522.91751335
ACGGGTT600.00203650722.91576616
AGCCGAC600.002037409722.9140230
TAGATCG600.002037409722.9140231
AGGTCGT1559.0221874E-1021.29002822
CAGAGTA69650.021.0458210
Produced by FastQC (version 0.11.5)