FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412089

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412089
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences354177
Sequences flagged as poor quality0
Sequence length61
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8680.24507520251173848No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAACC150.005780847754.99872619
GTGGTAT2700.043.8448261
TGGTATC3000.039.4380382
CGAATTA300.001922710636.67099815
GTATAAC350.004073689731.4633981
ACGGGAT1251.8189894E-1228.59933916
GTATCAA13200.028.364731
CGGGATG507.0231943E-427.49936717
GGTATCA6500.026.2598361
TATCAAC14650.025.9184822
AGAGTAC10850.025.34861611
CAGAGTA10900.025.2323410
CGACATT550.001225043124.99942221
CCGACAT550.001225043124.99942220
ATCAACG15200.024.6046943
TCAACGC15250.024.520564
CAACGCA15500.024.121665
AACGCAG16450.023.062866
TAGTACA600.002034709822.9128994
AGTACGG12450.022.09096113
Produced by FastQC (version 0.11.5)