FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412090

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412090
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15905
Sequences flagged as poor quality0
Sequence length91
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT1240.7796290474693492No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1110.6978937441056271No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT890.5595724614900974No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT560.35209053756680286No Hit
ATATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA470.2955045583149953No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT380.23891857906318767No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT380.23891857906318767No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT370.23263124803520907No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT340.2137692549512732No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA250.15718327569946558No Hit
GGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT250.15718327569946558No Hit
ATATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA240.15089594467148695No Hit
AGTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA230.14460861364350833No Hit
TATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT200.12574662055957245No Hit
GGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT190.11945928953159383No Hit
ACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT190.11945928953159383No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT180.11317195850361521No Hit
GTATCAACGCAGAGTACGGGGTATAAGACGAGCAAAAAAAAAAAAAAAAA170.1068846274756366No Hit
GTGGTATCAACGCAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTT170.1068846274756366No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG170.1068846274756366No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA160.10059729644765797No Hit
GTATCAACGCAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160.10059729644765797No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATCAA302.5299341E-670.833331
TACGAGT258.87832E-568.04
CTACGAG302.1880228E-456.6666643
TCGAGGT508.579955E-751.09
GTCGAGG551.655264E-646.363648
GTATCAA2050.045.6097531
TATCAAC2700.044.0740742
GGTATCA905.8935257E-1042.5000041
ATCAACG2800.040.9821433
TCAACGC2950.038.8983084
TGGTATC450.001614205237.777782
CAACGCA3100.037.0161325
AGTCGAG708.6879845E-636.4285747
AACGCAG3200.035.8593756
ACGCAGA3850.029.8051957
GCAGAGT3900.029.4230779
CGCAGAG3950.029.0506328
AGAGTCG2001.8189894E-1227.6255
CAGAGTC2053.6379788E-1226.9512184
AGGTTTT501.0246571E-425.512-13
Produced by FastQC (version 0.11.5)