FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412092

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412092
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences246192
Sequences flagged as poor quality0
Sequence length91
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT7320.2973289140183272No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6570.2668648859426789No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT5800.23558848378501335No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4030.1636933775264834No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAACGC150.001028377485.004463
TCTAACG150.001028377485.004462
ACGCCTA200.003219800863.7533536
AGTTAGC356.593351E-660.717482
TGGTATC2850.056.6696472
GTGGTAT2900.051.29581
TAACGCC250.007787399551.002684
ATACGGG551.7568345E-646.3660741
GGTATCA5800.041.7694361
GTATCAA11750.039.065881
TCGAGGT2150.035.5760429
GTCGAGG2300.035.103418
TATCAAC14100.034.6649442
ATCAACG14350.034.0610243
CGGAACC250.00278672734.00178516-17
GTCGAGA250.00278672734.00178582-83
TCAACGC14650.033.363534
CAACGCA14850.032.914195
GTTAGCT652.546772E-432.6940273
AACGCAG15950.030.6442436
Produced by FastQC (version 0.11.5)