FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005412094

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412094
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44826
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2910.6491768170258332No Hit
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT2510.559942890286887No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2240.49970998973809844No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1870.41716860750457324No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT1140.25431669120599654No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT1050.23423905768973366No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT930.2074688796680498No Hit
ATATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA890.19854548699415517No Hit
GTATGTAGGAGTTGAAGATTAGTCCGCCGTAGTCGGTGTACTCGTAGGTT810.18069870164636595No Hit
GGGTAAATACGGGCCCTATTTCAAAGATTTTTAGGGGAATTAATTCTAGG810.18069870164636595No Hit
CTCATGAGCTGTCCCCACATTAGGCTTAAAAACAGATGCAATTCCCGGAC800.1784678534778923No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA790.17623700530941866No Hit
CCTACATACTTCCCCCATTATTCCTAGAACCAGGCGACCTGCGACTCCTT750.16731361263552402No Hit
GCTCATGAGTGCAAGACGTCTTGTGATGTAATTATTATACGAATGGGGGC750.16731361263552402No Hit
GTATCAACGCAGAGTACGGGGTATAAGACGAGCAAAAAAAAAAAAAAAAA740.16508276446705036No Hit
GTCCCCACATTAGGCTTAAAAACAGATGCAATTCCCGGACGTCTAAACCA730.1628519162985767No Hit
TCCTAGTCCTGTATGCCCTTTTCCTAACACTCACAACAAAACTAACTAAT730.1628519162985767No Hit
GGACTAGGATGATGGCGGGCAGGATAGTTCAGACGGTTTCTATTTCCTGA730.1628519162985767No Hit
TCCTAGTCCTCATCGCCCTCCCATCCCTACGCATCCTTTACATAACAGAC720.16062106813010307No Hit
GTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA700.15615937179315575No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT650.1450051309507875No Hit
TATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT630.1405434346138402No Hit
GGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT630.1405434346138402No Hit
GTACCATTGGTGGCCAATTGATTTGATGGTAAGGGAGGGATCGTTGACCT590.13162004193994556No Hit
GGTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA560.12492749743452461No Hit
GGATAGTTCAGACGGTTTCTATTTCCTGAGCGTCTGAGATGTTAGTATTA550.12269664926605095No Hit
GTATCAACGCAGAGTACGGGATGGCACATGCAGCGCAAGTAGGTCTACAA540.12046580109757729No Hit
GGACTAGGAAGCAGATAAGGAAAATGATTATGAGGGCGTGATCATGAAAG530.11823495292910365No Hit
GTTCTAGGAATAATGGGGGAAGTATGTAGGAGTTGAAGATTAGTCCGCCG530.11823495292910365No Hit
GTGCAAGACGTCTTGTGATGTAATTATTATACGAATGGGGGCTTCAATCG510.11377325659215633No Hit
ATCATAGAAGAGCTTATCACCTTTCATGATCACGCCCTCATAATCATTTT510.11377325659215633No Hit
ATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT480.10708071208673536No Hit
GTTCAGTACCATTGGTGGCCAATTGATTTGATGGTAAGGGAGGGATCGTT470.10484986391826172No Hit
GTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCCT460.10261901574978807No Hit
CCAATGGTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCA460.10261901574978807No Hit
GTGTACTCGTAGGTTCAGTACCATTGGTGGCCAATTGATTTGATGGTAAG460.10261901574978807No Hit
ATGTAAAGGATGCGTAGGGATGGGAGGGCGATGAGGACTAGGATGATGGC450.10038816758131443No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)