FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412095

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412095
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44826
Sequences flagged as poor quality0
Sequence length61
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5241.1689644402801946No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1080.24093160219515458No Hit
GTATCAACGCAGAGTACGGGGTATAAGACGAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA960.21416142417347073No Hit
GTATGTAGGAGTTGAAGATTAGTCCGCCGTAGTCGGTGTACTCGTAGGTTCAGTACCATTG840.1873912461517869No Hit
GGGTAAATACGGGCCCTATTTCAAAGATTTTTAGGGGAATTAATTCTAGGACGATGGGCAT750.16731361263552402No Hit
CTCATGAGCTGTCCCCACATTAGGCTTAAAAACAGATGCAATTCCCGGACGTCTAAACCAA750.16731361263552402No Hit
TCCTAGTCCTGTATGCCCTTTTCCTAACACTCACAACAAAACTAACTAATACTAACATCTC710.1583902199616294No Hit
CCTACATACTTCCCCCATTATTCCTAGAACCAGGCGACCTGCGACTCCTTGACGTTGACAA690.1539285236246821No Hit
GTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA650.1450051309507875No Hit
GCTCATGAGTGCAAGACGTCTTGTGATGTAATTATTATACGAATGGGGGCTTCAATCGGGA630.1405434346138402No Hit
GGATAGTTCAGACGGTTTCTATTTCCTGAGCGTCTGAGATGTTAGTATTAGTTAGTTTTGT560.12492749743452461No Hit
GTATCAACGCAGAGTACGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTC550.12269664926605095No Hit
GGGGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCG530.11823495292910365No Hit
GAACTATCCTGCCCGCCATCATCCTAGTCCTCATCGCCCTCCCATCCCTACGCATCCTTTA520.11600410476062999No Hit
GGTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA500.11154240842368268No Hit
GTTCAGTACCATTGGTGGCCAATTGATTTGATGGTAAGGGAGGGATCGTTGACCTCGTCTG500.11154240842368268No Hit
GTCTTGCACTCATGAGCTGTCCCCACATTAGGCTTAAAAACAGATGCAATTCCCGGACGTC500.11154240842368268No Hit
GTTCTAGGAATAATGGGGGAAGTATGTAGGAGTTGAAGATTAGTCCGCCGTAGTCGGTGTA500.11154240842368268No Hit
GTCCCCACATTAGGCTTAAAAACAGATGCAATTCCCGGACGTCTAAACCAAACCACTTTCA500.11154240842368268No Hit
GGGTAGAGGGGGTGCTATAGGGTAAATACGGGCCCTATTTCAAAGATTTTTAGGGGAATTA490.10931156025520902No Hit
GTACCATTGGTGGCCAATTGATTTGATGGTAAGGGAGGGATCGTTGACCTCGTCTGTTATG480.10708071208673536No Hit
GGACTAGGATGATGGCGGGCAGGATAGTTCAGACGGTTTCTATTTCCTGAGCGTCTGAGAT450.10038816758131443No Hit
GGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATC450.10038816758131443No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTGGA150.00574752955.029
GAGCCTC150.00574752955.030
CTTAGTT150.00574752955.038
AGAGCCT150.00574752955.029
ACTTAGT150.00574752955.037
CCTCACT150.00574752955.033
GAGAGCC150.00574752955.028
ACGGGAT358.535014E-539.2857135
GTGGTAT1550.037.2580641
GGTTCAG300.001905962436.66666847
TGGTATC1650.035.02
GTCCGCC350.00405809731.42857222
CCGCCGT350.00405809731.42857224
TAGTCCG350.00405809731.42857220
ACGGGTA350.00405809731.4285725
AGGTTCA350.00405809731.42857246
TCCGCCG350.00405809731.42857223
TCGTAGG350.00405809731.42857242
GCCGTAG350.00405809731.42857226
AGTCCGC350.00405809731.42857221
Produced by FastQC (version 0.11.5)