FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412097

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412097
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1232540
Sequences flagged as poor quality0
Sequence length61
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG47220.38311129861911175No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT17600.035.6668431
TGGTATC19300.032.9290852
TCCGTCG400.00787037327.5021335
GTATCAA65350.026.331581
CGCGTCG550.001227211924.99787922
AGAGTAC40500.023.90017511
TATCAAC73850.023.3583412
AGACCGT600.002035656222.91658239
CGGTATA852.572201E-522.64513828
CGAATTA3050.022.5390715
CAGAGTA43900.022.29969610
TAGTCGG1254.6284185E-821.99813330
CGGGTAT1003.6208494E-621.9963517
GGTATCA33200.021.7272991
ATCAACG79550.021.712183
CGTAGAA2050.021.46159433
TCAACGC81350.021.2291774
ACGCTCG650.003239950421.15376938
ATACGTG1307.0078386E-821.1520525
CAACGCA82850.021.009085
Produced by FastQC (version 0.11.5)