FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412104

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412104
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences857481
Sequences flagged as poor quality0
Sequence length91
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26640.3106774377508073No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT24800.2892192363445954No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16530.19277395067645814No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGT13820.1611697518662221No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9930.11580431519765452No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT9690.11300541936206167No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT8820.10285942195803754No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT12400.048.677141
TACGTGC1450.046.8958476
AAGTACG2550.041.6733251
TGGTATC15650.041.5488932
ACGTGCA1650.038.633537
GGTATCA25350.035.7157671
GTATCAA47550.034.953011
ATACGTG1950.034.8712655
TATACGT1950.034.8712654
ATACGGG3050.034.8416371
ACAACGC500.002788799833.9994853
TCGAGGT10200.033.747529
TATCAAC57150.033.5384252
GTCGAGG11200.033.3904088
AGTCGAG14850.031.1929957
ATCAACG63500.030.1176573
CAACGCA65900.029.0852995
TCAACGC65950.029.0632464
AACGCAG66700.028.5452986
TATTACG905.1541487E-528.3329072
Produced by FastQC (version 0.11.5)