FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412105

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412105
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences857481
Sequences flagged as poor quality0
Sequence length61
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG34550.4029243796655552No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT14800.038.676261
ACCGAAC300.001925771736.6650350
TGGTATC16400.035.3857152
ATAGCTC601.7163529E-632.08377539
CGGTACC350.004095270831.4345145
ACGTTAG350.004096440531.43267342
TGTAACC350.00410112331.4253393
AATCGTC400.00787150427.50038128
GCACCGC400.00787374527.49877510
GTATCAA56950.026.0458131
GCCGCTA759.7432E-625.66851840
CGCGCCC651.0885451E-425.38496427
ATTAGAG651.08940854E-425.3820023
TCCAACG550.001226201825.00034324
CGTTCCC550.001226618924.99888617
TCTATAC901.4476427E-624.441933
AGAGTAC37450.023.64380611
TAATCGT701.7999348E-423.57175427
TATCAAC64700.023.0826112
GGTATCA28700.022.9170571
Produced by FastQC (version 0.11.5)