FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412110

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412110
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16587
Sequences flagged as poor quality0
Sequence length91
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGT216613.058419243986256No Hit
ATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTT5913.5630312895641163TruSeq Adapter, Index 11 (95% over 23bp)
ACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCT2571.5494061614517394RNA PCR Primer, Index 11 (96% over 25bp)
TTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTC1781.0731295592934227TruSeq Adapter, Index 11 (95% over 21bp)
GGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAA1400.8440344848375234No Hit
AGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAA1260.759631036353771No Hit
ATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAA1210.7294869476095738No Hit
AACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAAA1070.6450834991258214No Hit
TATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCT990.5968529571351058RNA PCR Primer, Index 11 (95% over 22bp)
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT810.48833423765599565No Hit
TCTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCG790.47627660215831674No Hit
CCGCGAGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGT700.4220172424187617No Hit
GGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAA660.39790197142340383No Hit
GATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAA620.37378670042804607No Hit
CAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAA620.37378670042804607No Hit
GTTGACTAGTACATGACCACTTCATACCTAACCGGTATACCCGTGGTGGA570.3436426116838488No Hit
GTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA550.3315849761861699No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCC530.319527340688491No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT480.2893832519442937No Hit
ATGCCGGCCAGAACCTGTTGGATCAACAACCCCAATCCCTGTCTCTTATA470.28335443419545425No Hit
TTTTTGGGACCATGCCGGCCAGAACCTGTTGGATCAACAACCCCAATCCA460.2773256164466148No Hit
TGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAA450.27129679869777534No Hit
GGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA420.253210345451257No Hit
GGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATCTGTCTCTTATA410.24718152770241758No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT410.24718152770241758No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACAGCTCGTATGCCGT380.22909507445589922No Hit
GCCCTATCCACCACGGGTATACCGGTTAGGTATGAAGTGGTCATGTACTA360.2170374389582203No Hit
GAGCGCCGCGAGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGC360.2170374389582203No Hit
GCGCCGCGAGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCAT350.21100862120938085No Hit
TTTTGGGACCATGCCGGCCAGAACCTGTTGGATCAACAACCCCAATCCAT350.21100862120938085No Hit
GGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAC330.19895098571170192No Hit
GGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAA320.1929221679628625No Hit
GATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAA300.18086453246518358No Hit
GGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAACT300.18086453246518358No Hit
CCGCGAGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCCTGTCTCT290.17483571471634413No Hit
GGCCAGAACCTGTTGGATCAACAACCCCAATCCATCTCGCGGCTGTCTCT270.16277807921866522No Hit
GGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCTGTCTC260.15674926146982576No Hit
AGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCCTGTCTCTTATAC260.15674926146982576No Hit
AGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATCTGTCTCT260.15674926146982576No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATGTCGTATGCCGT250.1507204437209863No Hit
ATGCCGGCCAGAACCTGTTGGATCAACAACCCCAATCCATCTCTGTCTCT240.14469162597214685No Hit
GTGTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGACGGAGCTA240.14469162597214685No Hit
GAGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCA240.14469162597214685No Hit
CTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTC240.14469162597214685TruSeq Adapter, Index 11 (95% over 21bp)
ATGCCGGCCAGAACCTGTTGGATCAACAACCCCAATCCATCTCGCGGCGC230.1386628082233074No Hit
GAGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATCTGTCTC230.1386628082233074No Hit
TTTTTGGGACCATGCCGGCCAGAACCTGTTGGATCAACAACCCCAATCCC230.1386628082233074No Hit
ATCCACCACGGGTATACCGGTTAGGTATGAAGTGGTCATGTACTAGTCAA230.1386628082233074No Hit
TTTTGGGACCATGCCGGCCAGAACCTGTTGGATCAACAACCCCAATCCCT230.1386628082233074No Hit
GTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAA220.13263399047446794No Hit
GGACCATGCCGGCCAGAACCTGTTGGATCAACAACCCCAATCCCTGTCTC210.1266051727256285No Hit
GGTATGAAGTTGACTAGTACATGACCACTTCATACCTAACCGGTATACCC210.1266051727256285No Hit
GCGAGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCC210.1266051727256285No Hit
ATGCCGGCCAGAACCTGTTGGATCAACAACCCCAATCCATCTCCTGTCTC210.1266051727256285No Hit
CAGCTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGC200.12057635497678906No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCGCGTATGCCGT200.12057635497678906No Hit
GACATATGATGCGGCCCTATCCACCACGGGTATACCGGTTAGGTATGAAG200.12057635497678906No Hit
CACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTG190.11454753722794961TruSeq Adapter, Index 11 (96% over 26bp)
GGCCAGAACCTGTTGGATCAACAACCCCAATCCATCTCTGTCTCTTATAC190.11454753722794961No Hit
GTATGAAGTTGACTAGTACATGACCACTTCATACCTAACCGGTATACCCG190.11454753722794961No Hit
TCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAA190.11454753722794961No Hit
GGCCAGAACCTGTTGGATCAACAACCCCAATCCCTGTCTCTTATACACAT190.11454753722794961No Hit
TTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAA190.11454753722794961No Hit
TTTTTGGGACCATGCCGGCCAGAACCTGTTGGATCAACAACCCCAATCCT180.10851871947911015No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT180.10851871947911015No Hit
GGACCATGCCGGCCAGAACCTGTTGGATCAACAACCCCAATCCATCTCGC180.10851871947911015No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACCTCTCGTATGCCGT180.10851871947911015No Hit
CCGCGAGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATCTG180.10851871947911015No Hit
GATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAA180.10851871947911015No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT180.10851871947911015No Hit
GGATTGGGGTTGTTGATCCAACAGGTTCTGGCCTGTCTCTTATACACATC170.10248990173027069No Hit
AGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAG170.10248990173027069No Hit
CTTCATACCTAACCGGTATACCCGTGGTGGATAGGGCCGCATCATATGTC170.10248990173027069No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGAGG354.7191902E-448.5092778
TCGAGGT354.7191902E-448.5092779
TTGGGAC409.1124716E-442.4456183
AGTCGAG450.001626330537.729447
AGATGGA1600.037.2524641
GTATCAA652.4215793E-432.749421
CAGAGTC1455.2750693E-1132.2001234
AGAGTCG1455.2750693E-1132.2001235
GATGGAT1850.032.121012
ACGCAGA1507.4578566E-1131.2211111
GCAGAGT1507.8216544E-1131.126793
CGCAGAG1507.8216544E-1131.126792
CCGCGAG703.7404578E-430.4101721
GCGAGAT703.8078902E-430.3182983
ATGGATT1850.029.826653
TATCAAC755.7038665E-428.2970812
CGAGATG755.7038665E-428.2970814
CGCGAGA755.7038665E-428.2970812
ACCATGC904.901652E-528.297088
GGACCAT904.901652E-528.297086
Produced by FastQC (version 0.11.5)