FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412121

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412121
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences173969
Sequences flagged as poor quality0
Sequence length61
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18721.0760537796963827No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2660.1529008041662595No Hit
GTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2190.12588449666319862No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACGTG150.00577216455.0077635
TACTATC150.00577873854.9919477
GTGGTAT3750.037.416041
GTGTCAC300.001917095436.6823921
TTAGAGT300.001919806136.671844
TGGTATC3850.036.4232372
CGAGGGT951.8189894E-1234.7417510
GCCCCGA501.8094195E-533.00465811
CCGAATT501.8094195E-533.00465814
TCGAGGG1003.6379788E-1233.0046589
GCTATCT350.004087330331.4330115
TTCCATA350.0040930931.4239732
ACAATCC350.0040930931.4239738
GTCGACA453.779303E-430.5598714
CGAATTA553.4706587E-530.00423615
GAGGGTT1101.2732926E-1130.00423611
GACTCAG553.4774836E-529.9956077
GGACTCA705.6642675E-627.5038836
CTAGGAT400.00784966327.5038834
GGTATCA8350.027.017571
Produced by FastQC (version 0.11.5)