FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005412122

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412122
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4639
Sequences flagged as poor quality0
Sequence length91
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT3026.510023712006897No Hit
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT671.4442767837896098TruSeq Adapter, Index 11 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCT591.271825824531149RNA PCR Primer, Index 11 (95% over 23bp)
GGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAA360.776029316663074No Hit
ATGCCGGCCAGAACCTGTTGGATCAACAACCCCAATCCCTGTCTCTTATA340.7329165768484587No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT310.6682474671265359No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCC300.6466910972192282No Hit
CCGCGAGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGT290.6251347273119207No Hit
CAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAA270.5820219874973055No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT270.5820219874973055No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT250.5389092476826902No Hit
GGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATCTGTCTCTTATA230.495796507868075No Hit
TTTTTGGGACCATGCCGGCCAGAACCTGTTGGATCAACAACCCCAATCCA230.495796507868075No Hit
AACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAAA230.495796507868075No Hit
ATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAA220.47424013796076736No Hit
GAGCGCCGCGAGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGC220.47424013796076736No Hit
AGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAA190.40957102823884456No Hit
GTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA180.388014658331537No Hit
GTATCAACGCAGAGTACGGGGACCATGCCGGCCAGAACCTGTTGGATCAA170.36645828842422934No Hit
GGCCAGAACCTGTTGGATCAACAACCCCAATCCATCTCGCGGCTGTCTCT160.34490191851692176No Hit
GGCCAGAACCTGTTGGATCAACAACCCCAATCCCTGTCTCTTATACACAT160.34490191851692176No Hit
GGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAC160.34490191851692176No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150.3233455486096141No Hit
GCGAGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCC140.30178917870230654No Hit
TTTTGGGACCATGCCGGCCAGAACCTGTTGGATCAACAACCCCAATCCAT140.30178917870230654No Hit
GGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA140.30178917870230654No Hit
GGATTGGGGTTGTTGATCCAACAGGTTCTGGCCCTGTCTCTTATACACAT140.30178917870230654No Hit
GTATCAACGCAGAGTACGGGACCATGCCGGCCAGAACCTGTTGGATCAAC130.2802328087949989No Hit
GGACCATGCCGGCCAGAACCTGTTGGATCAACAACCCCAATCCCTGTCTC130.2802328087949989No Hit
GTGTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGACCGTACTA130.2802328087949989No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG130.2802328087949989No Hit
GAGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCA130.2802328087949989No Hit
GCCAGAACCTGTTGGATCAACAACCCCAATCCCTGTCTCTTATACACATC120.2586764388876913No Hit
TTTTTGGGACCATGCCGGCCAGAACCTGTTGGATCAACAACCCCAATCCC120.2586764388876913No Hit
TTTTGGGACCATGCCGGCCAGAACCTGTTGGATCAACAACCCCAATCCCT120.2586764388876913No Hit
TTTTTGGGACCATGCCGGCCAGAACCTGTTGGATCTGTCTCTTATACACA110.23712006898038368No Hit
GATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAA110.23712006898038368No Hit
GATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAA110.23712006898038368No Hit
GGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAA110.23712006898038368No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG110.23712006898038368No Hit
GGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCTGTCTC110.23712006898038368No Hit
GCGCCGCGAGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCAT110.23712006898038368No Hit
GGCCAGAACCTGTTGGATCAACAACCCCAATCCATCTCTGTCTCTTATAC110.23712006898038368No Hit
AGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCCTGTCTCTTATAC100.21556369907307613No Hit
CCGCGAGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCCTGTCTCT100.21556369907307613No Hit
GGGTTGTTGATCCAACAGGTTCTGGCCGGCATCTGTCTCTTATACACATC100.21556369907307613No Hit
GGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCCGTACT100.21556369907307613No Hit
TATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100.21556369907307613No Hit
TATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCT90.1940073291657685No Hit
ATGCCGGCCAGAACCTGTTGGATCAACAACCCCAATCCATCTCTGTCTCT90.1940073291657685No Hit
TCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCG90.1940073291657685No Hit
ATGCCGGCCAGAACCTGTTGGATCAACAACCCCAATCCTGTCTCTTATAC90.1940073291657685No Hit
CACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTG80.17245095925846088TruSeq Adapter, Index 11 (95% over 24bp)
TGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAA80.17245095925846088No Hit
GGCCAGAACCTGTTGGATCAACAACCCCAATCCATCTCGCGGCGCTCCTG80.17245095925846088No Hit
TTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTC80.17245095925846088No Hit
ATTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA80.17245095925846088No Hit
GATTGGGGTTGTTGATCCAACAGGTTCTGGCCCTGTCTCTTATACACATC80.17245095925846088No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80.17245095925846088No Hit
GAGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATCTGTCTC80.17245095925846088No Hit
ATGCCGGCCAGAACCTGTTGGATCAACAACCCCTGTCTCTTATACACATC80.17245095925846088No Hit
GGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80.17245095925846088No Hit
TTTTTGGGACCATGCCGGCCAGAACCTGTTGGATCAACAACCCCTGTCTC80.17245095925846088No Hit
CGAGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCC70.15089458935115327No Hit
GGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70.15089458935115327No Hit
GGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAA70.15089458935115327No Hit
AGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCTG70.15089458935115327No Hit
CCGCGAGATGGATTGGGGTTGTTGATCCAACAGGTTCCTGTCTCTTATAC70.15089458935115327No Hit
ATGCCGGCCAGAACCTGTTGGATCAACAACCCCAATCCATCTCGCGGCTG70.15089458935115327No Hit
CAGCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGC70.15089458935115327No Hit
GCCAGAACCTGTTGGATCAACAACCCCAATCCATCTCGCGGCTGTCTCTT70.15089458935115327No Hit
AGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATCTGTCTCT70.15089458935115327No Hit
ATGCCGGCCAGAACCTGTTGGATCAACAACCCCCTGTCTCTTATACACAT60.12933821944384566No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT60.12933821944384566No Hit
GATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAA60.12933821944384566No Hit
GAGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCCTGTCTCTTATA60.12933821944384566No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT60.12933821944384566No Hit
GGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCCGTACTCTGCGT60.12933821944384566No Hit
GGCCAGAACCTGTTGGATCAACAACCCCAATCCATCCTGTCTCTTATACA60.12933821944384566No Hit
GATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATCTGTCTCTTATAC60.12933821944384566No Hit
GTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAA60.12933821944384566No Hit
AACAGGTTCTGGCCGGCATGGTCCCCGTACTCTGCGTTGATACCTGTCTC60.12933821944384566No Hit
TGCCGGCCAGAACCTGTTGGATCAACAACCCCAATCCCTGTCTCTTATAC60.12933821944384566No Hit
ATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAACTGTCTCTTATACACA60.12933821944384566No Hit
ATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAA60.12933821944384566No Hit
CCGCGAGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATCTG50.10778184953653806No Hit
GTTCTGGCCGGCATGGTCCCCGTACTCTGCGTTGATACCTGTCTCTTATA50.10778184953653806No Hit
GGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAGCAAA50.10778184953653806No Hit
GTTCTGGCCGGCATGGTCCCGTACTCTGCGTTGATACCTGTCTCTTATAC50.10778184953653806No Hit
AGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCCG50.10778184953653806No Hit
GATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATCTGTCTCTT50.10778184953653806No Hit
GATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCTGTCTCT50.10778184953653806No Hit
GGACCATGCCGGCCAGAACCTGTTGGATCAACAACCCCAATCCTGTCTCT50.10778184953653806No Hit
ATGCCGGCCAGAACCTGTTGGATCAACAACCCCAATCCATCCTGTCTCTT50.10778184953653806No Hit
GGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCGTACTC50.10778184953653806No Hit
TGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAA50.10778184953653806No Hit
GGACCATGCCGGCCAGAACCTGTTGGATCAACAACCCCAATCCATCTCGC50.10778184953653806No Hit
ATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG50.10778184953653806No Hit
GTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGACCGTACTAGA50.10778184953653806No Hit
ATCCAACAGGTTCTGGCCGGCATGGTCCCAAAACTGTCTCTTATACACAT50.10778184953653806No Hit
GAACCTGTTGGATCAACAACCCCAATCCATCTCGCGGCTGTCTCTTATAC50.10778184953653806No Hit
GAGCGCCGCGAGATGGATTGGGGTTGTTGATCCAACAGCTGTCTCTTATA50.10778184953653806No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)