FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005412123

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412123
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4639
Sequences flagged as poor quality0
Sequence length61
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG63413.666738521233023No Hit
GGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATCTGTCTCTTATACACATCTGACG310.6682474671265359No Hit
GGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAA250.5389092476826902No Hit
ATGCCGGCCAGAACCTGTTGGATCAACAACCCCAATCCCTGTCTCTTATACACATCTGACG220.47424013796076736No Hit
AGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAA180.388014658331537No Hit
CAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA170.36645828842422934No Hit
CCGCGAGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCCTGTCTCTTATACACATCT160.34490191851692176No Hit
GTATCAACGCAGAGTACGGGGACCATGCCGGCCAGAACCTGTTGGATCAACAACCCCAATC160.34490191851692176No Hit
TTTTTGGGACCATGCCGGCCAGAACCTGTTGGATCAACAACCCCAATCCCTGTCTCTTATA160.34490191851692176No Hit
GGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA150.3233455486096141No Hit
GGATTGGGGTTGTTGATCCAACAGGTTCTGGCCCTGTCTCTTATACACATCTGACGCTGCC150.3233455486096141No Hit
GTATCAACGCAGAGTACGGGACCATGCCGGCCAGAACCTGTTGGATCAACAACCCCAATCC140.30178917870230654No Hit
AACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA140.30178917870230654No Hit
GGCCAGAACCTGTTGGATCAACAACCCCAATCCCTGTCTCTTATACACATCTGACGCTGCC130.2802328087949989No Hit
GGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCTGTCTCTTATACACATC130.2802328087949989No Hit
GGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAACTGTCTCTTATA120.2586764388876913No Hit
GGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAACTGTCTCTTATAC120.2586764388876913No Hit
GTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA110.23712006898038368No Hit
ATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAACTGTCTCTTATACACATCTGACGCTGC110.23712006898038368No Hit
AGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCCTGTCTCTTATACACATCTGACGC110.23712006898038368No Hit
GAGCGCCGCGAGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAA100.21556369907307613No Hit
GGCCAGAACCTGTTGGATCAACAACCCCAATCCATCTCGCGGCTGTCTCTTATACACATCT100.21556369907307613No Hit
ATGCCGGCCAGAACCTGTTGGATCAACAACCCCTGTCTCTTATACACATCTGACGCTGCCG90.1940073291657685No Hit
GATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATCTGTCTCTTATACACATCTGACGC90.1940073291657685No Hit
GGATTGGGGTTGTTGATCCAACAGGTTCTGGCCTGTCTCTTATACACATCTGACGCTGCCG90.1940073291657685No Hit
GGGTTGTTGATCCAACAGGTTCTGGCCGGCATCTGTCTCTTATACACATCTGACGCTGCCG90.1940073291657685No Hit
GATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAA90.1940073291657685No Hit
ATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA80.17245095925846088No Hit
GGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAA80.17245095925846088No Hit
AGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATCTGTCTCTTATACACATCT80.17245095925846088No Hit
GGCCAGAACCTGTTGGATCAACAACCCCAATCCATCTCTGTCTCTTATACACATCTGACGC80.17245095925846088No Hit
GGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA70.15089458935115327No Hit
GGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAACTGTCTCTTATACACATC70.15089458935115327No Hit
GGACCATGCCGGCCAGAACCTGTTGGATCAACAACCCCAATCCATCTCTGTCTCTTATACA70.15089458935115327No Hit
GGCCAGAACCTGTTGGATCAACAACCCCAATCCTGTCTCTTATACACATCTGACGCTGCCG70.15089458935115327No Hit
ATGCCGGCCAGAACCTGTTGGATCAACAACCCCAATCCATCTCTGTCTCTTATACACATCT70.15089458935115327No Hit
CCGCGAGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATCTGTCTCTTATACA70.15089458935115327No Hit
GGACCATGCCGGCCAGAACCTGTTGGATCAACAACCCCAATCCCTGTCTCTTATACACATC70.15089458935115327No Hit
GGCCAGAACCTGTTGGATCAACAACCCCAATCCATCTCCTGTCTCTTATACACATCTGACG60.12933821944384566No Hit
CCGCGAGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAA60.12933821944384566No Hit
GATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCCTGTCTCTTATACACATCTGACGCT60.12933821944384566No Hit
ATGCCGGCCAGAACCTGTTGGATCAACAACCCCAATCCATCTCCTGTCTCTTATACACATC60.12933821944384566No Hit
CCGCGAGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCTGTCTCTTATACACATCTG60.12933821944384566No Hit
GATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.12933821944384566No Hit
GAGCGCCGCGAGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCCTGTCTCTTATACA60.12933821944384566No Hit
GGGGTTGTTGATCCAACAGGTTCTGGCCGGCATCTGTCTCTTATACACATCTGACGCTGCC60.12933821944384566No Hit
TTTTTGGGACCATGCCGGCCAGAACCTGTTGGATCTGTCTCTTATACACATCTGACGCTGC60.12933821944384566No Hit
GTATCAACGCAGAGTACGGGGACCATGCCGGCCAGAACCTGTTCTGTCTCTTATACACATC60.12933821944384566No Hit
ATGCCGGCCAGAACCTGTTGGATCAACAACCCCAATCCTGTCTCTTATACACATCTGACGC60.12933821944384566No Hit
GAACCTGTTGGATCAACAACCCCAATCCATCTCGCGGCTGTCTCTTATACACATCTGACGC60.12933821944384566No Hit
GGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCACTGTCTCTTATACACATCTGACGC60.12933821944384566No Hit
ATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCCTGTCTCTTATACACATCTGACGCTG50.10778184953653806No Hit
GAGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAACTGTCTCT50.10778184953653806No Hit
GTATCAACGCAGAGTACGGGGACCATGCCGGCCAGAACCTGTCTCTTATACACATCTGACG50.10778184953653806No Hit
GATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAA50.10778184953653806No Hit
GATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCTGTCTCTTATACACATCT50.10778184953653806No Hit
ATGCCGGCCAGAACCTGTTGGATCAACAACCCCAATCCATCCTGTCTCTTATACACATCTG50.10778184953653806No Hit
GTATCAACGCAGAGTACGGGACCATGCCGGCCAGAACCTGTCTCTTATACACATCTGACGC50.10778184953653806No Hit
GGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCGTACTCTGCGTTGATAC50.10778184953653806No Hit
GAGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAACTGTCTC50.10778184953653806No Hit
CTGTTGGATCAACAACCCCAATCCATCTCGCGGCGCTCCTGTCTCTTATACACATCTGACG50.10778184953653806No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)