FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412126

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412126
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5245
Sequences flagged as poor quality0
Sequence length91
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT4759.05624404194471No Hit
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT881.6777883698760723TruSeq Adapter, Index 11 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCT861.6396568160152527RNA PCR Primer, Index 11 (95% over 23bp)
GGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAA551.0486177311725453No Hit
TTTTTGGGACCATGCCGGCCAGAACCTGTTGGATCAACAACCCCAATCCA450.8579599618684461No Hit
AGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAA450.8579599618684461No Hit
ATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAA410.7816968541468066No Hit
AACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAAA370.7054337464251669No Hit
GGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATCTGTCTCTTATA350.667302192564347No Hit
GAGCGCCGCGAGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGC350.667302192564347No Hit
TTTTGGGACCATGCCGGCCAGAACCTGTTGGATCAACAACCCCAATCCAT310.5910390848427073No Hit
CCGCGAGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGT280.5338417540514776No Hit
GTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA270.5147759771210677No Hit
CAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAA270.5147759771210677No Hit
GATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAA260.4957102001906578No Hit
GGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAC250.47664442326024786No Hit
GGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAACT250.47664442326024786No Hit
ATGCCGGCCAGAACCTGTTGGATCAACAACCCCAATCCCTGTCTCTTATA230.43851286939942796No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT230.43851286939942796No Hit
GGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAA230.43851286939942796No Hit
GCGAGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCC210.4003813155386082No Hit
TTTTTGGGACCATGCCGGCCAGAACCTGTTGGATCAACAACCCCAATCCC200.3813155386081983No Hit
TATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCT200.3813155386081983No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCC200.3813155386081983No Hit
GGACCATGCCGGCCAGAACCTGTTGGATCAACAACCCCAATCCCTGTCTC190.3622497616777884No Hit
GGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA190.3622497616777884No Hit
GGATTGGGGTTGTTGATCCAACAGGTTCTGGCCCTGTCTCTTATACACAT190.3622497616777884No Hit
GATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAACT180.34318398474737843No Hit
GAGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCA180.34318398474737843No Hit
GGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCTGTCTC180.34318398474737843No Hit
TTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTC170.32411820781696854No Hit
TTTTGGGACCATGCCGGCCAGAACCTGTTGGATCAACAACCCCAATCCCT170.32411820781696854No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT170.32411820781696854No Hit
GCGCCGCGAGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCAT160.3050524308865586No Hit
TCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCG150.2859866539561487No Hit
GGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAACTGTCTCT150.2859866539561487No Hit
GGCCAGAACCTGTTGGATCAACAACCCCAATCCATCTCTGTCTCTTATAC150.2859866539561487No Hit
AGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCCTGTCTCTTATAC140.2669208770257388No Hit
GCCAGAACCTGTTGGATCAACAACCCCAATCCCTGTCTCTTATACACATC140.2669208770257388No Hit
GGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAACTGTCTC140.2669208770257388No Hit
ATGCCGGCCAGAACCTGTTGGATCAACAACCCCAATCCATCTCTGTCTCT130.2478551000953289No Hit
TTTTTGGGACCATGCCGGCCAGAACCTGTTGGATCAACAACCCCTGTCTC130.2478551000953289No Hit
GGATTGGGGTTGTTGATCCAACAGGTTCTGGCCTGTCTCTTATACACATC120.22878932316491898No Hit
GGCCAGAACCTGTTGGATCAACAACCCCAATCCCTGTCTCTTATACACAT120.22878932316491898No Hit
ATGCCGGCCAGAACCTGTTGGATCAACAACCCCAATCCATCTCGCGGCGC120.22878932316491898No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120.22878932316491898No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGCTCTCGTATGCCGT110.20972354623450903No Hit
CCGCGAGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATCTG110.20972354623450903No Hit
GGCCAGAACCTGTTGGATCAACAACCCCAATCCTGTCTCTTATACACATC110.20972354623450903No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGAGCTCGTATGCCGT110.20972354623450903No Hit
GATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAA110.20972354623450903No Hit
TTTTGGGACCATGCCGGCCAGAACCTGTTGGATCAACAACCCCTGTCTCT110.20972354623450903No Hit
GAGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATCTGTCTC110.20972354623450903No Hit
ATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAA110.20972354623450903No Hit
GGCCAGAACCTGTTGGATCAACAACCCCAATCCATCTCCTGTCTCTTATA100.19065776930409914No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATGTCGTATGCCGT100.19065776930409914No Hit
GGACCATGCCGGCCAGAACCTGTTGGATCAACAACCCCAATCCATCTCTG100.19065776930409914No Hit
ATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAACTGTCTCTTATACACA100.19065776930409914No Hit
AGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATCTGTCTCT100.19065776930409914No Hit
ATGCCGGCCAGAACCTGTTGGATCAACAACCCCAATCCTGTCTCTTATAC100.19065776930409914No Hit
GATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAACTG100.19065776930409914No Hit
GGCCAGAACCTGTTGGATCAACAACCCCAATCCATCTCGCGGCTGTCTCT90.17159199237368922No Hit
ATGCCGGCCAGAACCTGTTGGATCAACAACCCCAATCCATCTCCTGTCTC90.17159199237368922No Hit
GAGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCCTGTCTCTTATA90.17159199237368922No Hit
TTTTTGGGACCATGCCGGCCAGAACCTGTTGGATCAACAACCCCCTGTCT90.17159199237368922No Hit
GGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAACTGTCT90.17159199237368922No Hit
GATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCTGTCTCT90.17159199237368922No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG90.17159199237368922No Hit
GATTGGGGTTGTTGATCCAACAGGTTCTGGCCCTGTCTCTTATACACATC90.17159199237368922No Hit
TGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAA90.17159199237368922No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG90.17159199237368922No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90.17159199237368922No Hit
TCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAA90.17159199237368922No Hit
CACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTG80.1525262154432793TruSeq Adapter, Index 11 (95% over 24bp)
TTTTTGGGACCATGCCGGCCAGAACCTGTTGGATCTGTCTCTTATACACA80.1525262154432793No Hit
TGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAA80.1525262154432793No Hit
ATGCCGGCCAGAACCTGTTGGATCAACAACCCCAATCCATCTCGCCTGTC80.1525262154432793No Hit
TTTTGGGACCATGCCGGCCAGAACCTGTTGGATCAACAACCCCCTGTCTC80.1525262154432793No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATATCGTATGCCGT80.1525262154432793No Hit
GATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATCTGTCTCTTATAC80.1525262154432793No Hit
CCGCGAGATGGATTGGGGTTGTTGATCCAACAGGTTCCTGTCTCTTATAC80.1525262154432793No Hit
GTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAA80.1525262154432793No Hit
CAGCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGC80.1525262154432793No Hit
TTTTGGGACCATGCCGGCCAGAACCTGTTGGATCTGTCTCTTATACACAT80.1525262154432793No Hit
ATGCCGGCCAGAACCTGTTGGATCAACAACCCCTGTCTCTTATACACATC80.1525262154432793No Hit
TTTTGGGACCATGCCGGCCAGAACCTGTTGGATCAACAACCCCAATCCTG80.1525262154432793No Hit
GACCATGCCGGCCAGAACCTGTTGGATCAACAACCCCAATCCCTGTCTCT70.1334604385128694No Hit
TTTTTGGGACCATGCCGGCCAGAACCTGTTGGATCAACAACCCCAATCCT70.1334604385128694No Hit
ATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG70.1334604385128694No Hit
GATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAACTGTCTCTTATACAC70.1334604385128694No Hit
GGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAA70.1334604385128694No Hit
AGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCTG70.1334604385128694No Hit
ATTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA70.1334604385128694No Hit
GGACCATGCCGGCCAGAACCTGTTGGATCAACAACCCCAATCCTGTCTCT70.1334604385128694No Hit
GAGCGCCGCGAGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCCTG70.1334604385128694No Hit
ATGCCGGCCAGAACCTGTTGGATCAACAACCCCAATCCATCTCGCGGCTG70.1334604385128694No Hit
GGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCTGTCTCT70.1334604385128694No Hit
TTTTTTGGGACCATGCCGGCCAGAACCTGTTGGATCAACAACCCCAATCC70.1334604385128694No Hit
GGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAGCAAA60.11439466158245949No Hit
CTGTTGGATCAACAACCCCAATCCATCTCGCGGCGCTCCTGTCTCTTATA60.11439466158245949No Hit
GGGTTGTTGATCCAACAGGTTCTGGCCGGCATCTGTCTCTTATACACATC60.11439466158245949No Hit
AGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAG60.11439466158245949No Hit
ATCCAACAGGTTCTGGCCGGCATGGTCCCAGCAAAAAAAAAAAAAAAAAA60.11439466158245949No Hit
TGCTGGGACCATGCCGGCCAGAACCTGTTGGATCAACAACCCCAATCCAT60.11439466158245949No Hit
ATCCAACAGGTTCTGGCCGGCATGGTCCCAAAACTGTCTCTTATACACAT60.11439466158245949No Hit
GGGGTTGTTGATCCAACAGGTTCTGGCCGGCATCTGTCTCTTATACACAT60.11439466158245949No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGGGAC507.495328E-750.9999963
TGGGACC507.495328E-750.9999964
GGGACCA551.4466768E-646.3636325
TTTGGGA551.4466768E-646.3636322
GGACCAT752.5093505E-739.6666646
ACCATGC856.6994835E-735.08
GACCATG856.6994835E-735.07
ACGCAGA500.002542573533.9999961
CAGAGTC500.002542573533.9999964
AGAGTCG500.002542573533.9999965
GCAGAGT500.002542573533.9999963
CGCAGAG500.002542573533.9999962
GAGATGG500.002542573533.9999961
GGATTGG1154.8676156E-933.2608681
GATGGAT652.2225337E-432.6923073
CCATGCC951.5999158E-631.3157869
AGATGGA550.00405687130.909092
ATTGGGG1251.0988515E-830.5999983
GATTGGG1251.0988515E-830.5999982
TTGGGGT1251.0988515E-830.5999984
Produced by FastQC (version 0.11.5)