FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005412127

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412127
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5245
Sequences flagged as poor quality0
Sequence length61
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG91717.483317445185893No Hit
ATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA310.5910390848427073No Hit
ATGCCGGCCAGAACCTGTTGGATCAACAACCCCAATCCCTGTCTCTTATACACATCTGACG300.5719733079122974No Hit
GGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAA290.5529075309818875No Hit
TTTTTGGGACCATGCCGGCCAGAACCTGTTGGATCAACAACCCCAATCCCTGTCTCTTATA240.45757864632983797No Hit
AACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA230.43851286939942796No Hit
TTTTGGGACCATGCCGGCCAGAACCTGTTGGATCAACAACCCCAATCCCTGTCTCTTATAC230.43851286939942796No Hit
GTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA210.4003813155386082No Hit
GGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATCTGTCTCTTATACACATCTGACG210.4003813155386082No Hit
GGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAA200.3813155386081983No Hit
GGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAACTGTCTCTTATA190.3622497616777884No Hit
GGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCTGTCTCTTATACACATC180.34318398474737843No Hit
GGCCAGAACCTGTTGGATCAACAACCCCAATCCCTGTCTCTTATACACATCTGACGCTGCC170.32411820781696854No Hit
TTTTTGGGACCATGCCGGCCAGAACCTGTTGGATCAACAACCCCAATCCTGTCTCTTATAC170.32411820781696854No Hit
GGACCATGCCGGCCAGAACCTGTTGGATCAACAACCCCAATCCCTGTCTCTTATACACATC170.32411820781696854No Hit
GGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAACTGTCTCTTATAC160.3050524308865586No Hit
TTTTGGGACCATGCCGGCCAGAACCTGTTGGATCAACAACCCCTGTCTCTTATACACATCT150.2859866539561487No Hit
CAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA140.2669208770257388No Hit
GGATTGGGGTTGTTGATCCAACAGGTTCTGGCCTGTCTCTTATACACATCTGACGCTGCCG140.2669208770257388No Hit
AGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAA130.2478551000953289No Hit
GGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAACTGTCTCTTATACACATC130.2478551000953289No Hit
AGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATCTGTCTCTTATACACATCT130.2478551000953289No Hit
AGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCCTGTCTCTTATACACATCTGACGC130.2478551000953289No Hit
GAGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAACTGTCTC130.2478551000953289No Hit
GGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA120.22878932316491898No Hit
TTTTTGGGACCATGCCGGCCAGAACCTGTTGGATCAACAACCCCTGTCTCTTATACACATC120.22878932316491898No Hit
GAGCGCCGCGAGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAA120.22878932316491898No Hit
GGATTGGGGTTGTTGATCCAACAGGTTCTGGCCCTGTCTCTTATACACATCTGACGCTGCC120.22878932316491898No Hit
GGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAA120.22878932316491898No Hit
GATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAA120.22878932316491898No Hit
GATTGGGGTTGTTGATCCAACAGGTTCTGGCCCTGTCTCTTATACACATCTGACGCTGCCG110.20972354623450903No Hit
ATGCCGGCCAGAACCTGTTGGATCAACAACCCCAATCCATCTCCTGTCTCTTATACACATC110.20972354623450903No Hit
GGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAACTGTCTCTTATACACATCT110.20972354623450903No Hit
GATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA110.20972354623450903No Hit
ATGCCGGCCAGAACCTGTTGGATCAACAACCCCAATCCATCTCGCGGCGCTCCTGTCTCTT110.20972354623450903No Hit
GGCCAGAACCTGTTGGATCAACAACCCCAATCCATCTCTGTCTCTTATACACATCTGACGC110.20972354623450903No Hit
TTTTGGGACCATGCCGGCCAGAACCTGTTGGATCAACAACCCCAATCCATCTCTGTCTCTT100.19065776930409914No Hit
ATGCCGGCCAGAACCTGTTGGATCAACAACCCCAATCCATCTCGCGGCTGTCTCTTATACA100.19065776930409914No Hit
TTTTGGGACCATGCCGGCCAGAACCTGTTGGATCAACAACCCCAATCCTGTCTCTTATACA100.19065776930409914No Hit
GATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATCTGTCTCTTATACACATCTGACGC100.19065776930409914No Hit
CCGCGAGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCCTGTCTCTTATACACATCT100.19065776930409914No Hit
GCCAGAACCTGTTGGATCAACAACCCCAATCCCTGTCTCTTATACACATCTGACGCTGCCG100.19065776930409914No Hit
GAGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCCTGTCTCTTATACACATCTGACG90.17159199237368922No Hit
GGCCAGAACCTGTTGGATCAACAACCCCAATCCTGTCTCTTATACACATCTGACGCTGCCG90.17159199237368922No Hit
ATGCCGGCCAGAACCTGTTGGATCAACAACCCCAATCCATCTCTGTCTCTTATACACATCT90.17159199237368922No Hit
GGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAACTGTCTCTTATACACATC90.17159199237368922No Hit
TTTTTGGGACCATGCCGGCCAGAACCTGTTGGATCTGTCTCTTATACACATCTGACGCTGC90.17159199237368922No Hit
GAGCGCCGCGAGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATCTGTCTCTT90.17159199237368922No Hit
GGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAACTGTCTCTTATACACAT90.17159199237368922No Hit
GGGTTGTTGATCCAACAGGTTCTGGCCGGCATCTGTCTCTTATACACATCTGACGCTGCCG90.17159199237368922No Hit
GAGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATCTGTCTCTTATACACATC90.17159199237368922No Hit
AGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCTGTCTCTTATACA90.17159199237368922No Hit
GGCCAGAACCTGTTGGATCAACAACCCCAATCCATCTCCTGTCTCTTATACACATCTGACG80.1525262154432793No Hit
GGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA80.1525262154432793No Hit
GTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAAA80.1525262154432793No Hit
ATCCAACAGGTTCTGGCCGGCATGGTCCCAAAACTGTCTCTTATACACATCTGACGCTGCC80.1525262154432793No Hit
TTTTTGGGACCATGCCGGCCAGAACCTGTTGGATCAACAACCCCCTGTCTCTTATACACAT80.1525262154432793No Hit
GATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCTGTCTCTTATACACATCT80.1525262154432793No Hit
AGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAACTGTCTCT80.1525262154432793No Hit
GATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAACTGTCTCTTATACA80.1525262154432793No Hit
GAACCTGTTGGATCAACAACCCCAATCCATCTCGCGGCTGTCTCTTATACACATCTGACGC80.1525262154432793No Hit
GGACCATGCCGGCCAGAACCTGTTGGATCAACAACCCCAATCCATCTCTGTCTCTTATACA70.1334604385128694No Hit
TTTTTGGGACCATGCCGGCCAGAACCTGTTGGATCCTGTCTCTTATACACATCTGACGCTG70.1334604385128694No Hit
GGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCTGTCTCTTATACACATCT70.1334604385128694No Hit
GTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAA70.1334604385128694No Hit
ATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAACTGTCTCTTATACACATCTGACGCTGC70.1334604385128694No Hit
TTTTTGGGACCATGCCGGCCAGAACCTGTTGGATCAACAACCCCAATCCATCTCGCGGCGC70.1334604385128694No Hit
GCGAGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATCTGTCTCTTATACACA70.1334604385128694No Hit
GGACCATGCCGGCCAGAACCTGTTGGATCAACAACCCCAATCCTGTCTCTTATACACATCT70.1334604385128694No Hit
ATGCCGGCCAGAACCTGTTGGATCAACAACCCCAATCCTGTCTCTTATACACATCTGACGC70.1334604385128694No Hit
GGCCAGAACCTGTTGGATCAACAACCCCAATCCATCTCGCGGCGCTCCTGTCTCTTATACA70.1334604385128694No Hit
GATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAACTGTCTCTTATAC60.11439466158245949No Hit
ATGCCGGCCAGAACCTGTTGGATCAACAACCCCCTGTCTCTTATACACATCTGACGCTGCC60.11439466158245949No Hit
GAGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAACTGTCTCT60.11439466158245949No Hit
TTTTTGGGACCATGCCGGCCAGAACCTGTTGGATCAACAACCCCAATCCATCTCTGTCTCT60.11439466158245949No Hit
ATGCCGGCCAGAACCTGTTGGATCAACAACCCCTGTCTCTTATACACATCTGACGCTGCCG60.11439466158245949No Hit
AGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAACTGTCTCTT60.11439466158245949No Hit
TGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA60.11439466158245949No Hit
GGCCAGAACCTGTTGGATCAACAACCCCAATCCATCTCGCGGCTGTCTCTTATACACATCT60.11439466158245949No Hit
TTTTGGGACCATGCCGGCCAGAACCTGTTGGATCTGTCTCTTATACACATCTGACGCTGCC60.11439466158245949No Hit
GTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.11439466158245949No Hit
CCGCGAGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATCTGTCTCTTATACA60.11439466158245949No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)