FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412129

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412129
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44356
Sequences flagged as poor quality0
Sequence length61
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10062.2680133465596537No Hit
GTATCAACGCAGAGTACGGGGTATAAGACGAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA640.14428713139146904No Hit
GTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA490.11046983497159348No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTTATA150.005722439455.0598222
TAAGTTG150.0057480854.9977465
GTATCAA3750.036.7065471
TGGTATC851.8189894E-1135.626942
GTGGTAT851.8189894E-1135.626941
TATCAAC4350.032.2764472
CAGAGTA2200.029.9987710
AGAGTAC2200.029.9987711
ATCAACG4950.028.3321723
TCAACGC5050.027.7711374
ACGGGGA705.548296E-627.49887316
CAACGCA5150.027.2318955
AACGCAG5200.026.4412236
GGTATCA1251.6334525E-924.1990053
AGTACGG2750.023.9990213
GAGTACG2800.023.57046312
GCAGAGT6100.022.540069
TACGGGA1103.143232E-722.49907715
ACGCAGA6150.022.3568087
CGCAGAG6150.022.3568088
Produced by FastQC (version 0.11.5)