FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412133

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412133
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4474
Sequences flagged as poor quality0
Sequence length61
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG72516.20473848904783No Hit
GGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAA481.0728654447921324No Hit
ATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA280.6258381761287439No Hit
GGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAA260.581135449262405No Hit
CAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA200.44702726866338843No Hit
GTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA180.4023245417970496No Hit
GGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATCTGTCTCTTATACACATCTGACG150.33527045149754137No Hit
AGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAA140.31291908806437196No Hit
GGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAA140.31291908806437196No Hit
GGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA130.2905677246312025No Hit
GTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAAA130.2905677246312025No Hit
AACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA130.2905677246312025No Hit
TGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA130.2905677246312025No Hit
GATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAA130.2905677246312025No Hit
ATGCCGGCCAGAACCTGTTGGATCAACAACCCCAATCCCTGTCTCTTATACACATCTGACG120.2682163611980331No Hit
GAGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAA100.22351363433169422No Hit
GTATCAACGCAGAGTACGGGACCATGCCGGCCAGAACCTGTTGGATCAACAACCCCAATCC100.22351363433169422No Hit
TTTTTGGGACCATGCCGGCCAGAACCTGTTGGATCAACAACCCCAATCCCTGTCTCTTATA90.2011622708985248No Hit
GAGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATCTGTCTCTTATACACATC90.2011622708985248No Hit
GGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCCGTACTCTGCGTTGATA80.17881090746535538No Hit
GCGAGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATCTGTCTCTTATACACA80.17881090746535538No Hit
GGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAACTGTCTCTTATA80.17881090746535538No Hit
GGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAACTGTCTCTTATAC80.17881090746535538No Hit
GATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA80.17881090746535538No Hit
ATGCCGGCCAGAACCTGTTGGATCAACAACCCCAATCCATCTCTGTCTCTTATACACATCT80.17881090746535538No Hit
GGATTGGGGTTGTTGATCCAACAGGTTCTGGCCCTGTCTCTTATACACATCTGACGCTGCC70.15645954403218598No Hit
GATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAA70.15645954403218598No Hit
GAGCGCCGCGAGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAA70.15645954403218598No Hit
GTATCAACGCAGAGTACGGGGACCATGCCGGCCAGAACCTGTTGGATCAACAACCCCAATC70.15645954403218598No Hit
TTTTGGGACCATGCCGGCCAGAACCTGTTGGATCAACAACCCCTGTCTCTTATACACATCT70.15645954403218598No Hit
GCGAGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAA60.13410818059901655No Hit
ATGCCGGCCAGAACCTGTTGGATCAACAACCCCAATCCATCTCCTGTCTCTTATACACATC60.13410818059901655No Hit
ATCCAACAGGTTCTGGCCGGCATGGTCCCAAAACTGTCTCTTATACACATCTGACGCTGCC60.13410818059901655No Hit
GGACCATGCCGGCCAGAACCTGTTGGATCAACAACCCCAATCCCTGTCTCTTATACACATC60.13410818059901655No Hit
GTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.13410818059901655No Hit
GAGTAAAAGGGTTCATTCGTGTCGTTTCTGTCTTTCTTTCTTTCTTTCTTTCTTCAGCCTT50.11175681716584711No Hit
TCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.11175681716584711No Hit
GGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCAAAAAAAAAAAAAAAAAAAAAAAAA50.11175681716584711No Hit
AGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCCTGTCTCTTATACACATCTGACGC50.11175681716584711No Hit
GATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCTGTCTCTTATACACATCT50.11175681716584711No Hit
ATGCCGGCCAGAACCTGTTGGATCAACAACCCCTGTCTCTTATACACATCTGACGCTGCCG50.11175681716584711No Hit
GACCATGCCGGCCAGAACCTGTTGGATCAACAACCCCAATCCCTGTCTCTTATACACATCT50.11175681716584711No Hit
GAGCGCCGCGAGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATCTGTCTCTT50.11175681716584711No Hit
GGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAACTGTCTCTTATACACAT50.11175681716584711No Hit
GCCAGAACCTGTTGGATCAACAACCCCAATCCTGTCTCTTATACACATCTGACGCTGCCGA50.11175681716584711No Hit
AGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATCTGTCTCTTATACACATCT50.11175681716584711No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC150.00541358755.02
GTGGTAT150.00541358755.01
GTATCAA705.0931703E-1139.2857131
TATCAAC705.0931703E-1139.2857132
AACGCAG651.202352E-938.0769236
CAACGCA651.202352E-938.0769235
TGCCGGC300.00172704636.6666682
GCCGGCC300.00172704636.6666683
ATGCCGG300.00172704636.6666681
CGGCCAG300.00172704636.6666685
CCGGCCA300.00172704636.6666684
ATCAACG751.07320375E-1036.6666643
TCAACGC702.4847395E-935.3571434
GGCCAGA350.003679908631.4285726
ACGCAGA809.169526E-930.9375027
CGCAGAG809.169526E-930.9375028
CAGAGTC851.6569174E-829.11764710
GCAGAGT851.6569174E-829.1176479
CCTGTTG400.007072895827.514
CCAGAAC400.007072895827.58
Produced by FastQC (version 0.11.5)