FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412134

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412134
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35183
Sequences flagged as poor quality0
Sequence length91
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2960.8413154080095501No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2180.6196174288718983No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT1460.4149731404371429No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1460.4149731404371429No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1090.30980871443594915No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT910.2586476423272603No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT810.23022482448909984No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT740.21032885200238752No Hit
GGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT590.1676946252451468No Hit
ATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT540.15348321632606657No Hit
ACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT480.1364295256231703No Hit
GGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT470.13358724383935422No Hit
TATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT440.1250603984879061No Hit
GTATCAACGCAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT370.10516442600119376No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT705.4569682E-1154.642861
AAGTACG250.007733894551.01
GTATCAA4750.048.315791
TGGTATC802.0008883E-1047.8125042
GGTATCA2150.045.4651151
TATCAAC5550.043.648652
GTCGAGG701.8297942E-742.5000048
TCGAGGT701.8297942E-742.5000049
TCAACGC6100.040.4098364
ATCAACG6200.039.7580643
CAACGCA6300.039.1269845
AACGCAG6300.039.1269846
ACGGGAC359.125293E-636.42857416-17
ACGGGCA301.5803629E-435.41666816-17
CGCAGAG7250.034.586218
GCAGAGT7350.034.1156469
ACGCAGA7250.034.07
GTATAAG550.00439017730.909092
AGTCGAG1101.776607E-730.909097
CAGAGTA4700.028.93617210-11
Produced by FastQC (version 0.11.5)