FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412135

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412135
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35183
Sequences flagged as poor quality0
Sequence length61
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3480.9891140607679846No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGGGC303.4221703E-545.83333216
GTGGTAT850.042.0588231
ACGGGAA358.497379E-539.28571316
TCGGTCT300.001900370836.66666815
GCTATCG300.001900370836.66666811
TCGCTAT300.001900370836.6666689
CGGTCTC300.001900370836.66666816
GTATCAA5800.034.6120721
ACTTGTT401.8644106E-434.3749963
GTACTTG401.8644106E-434.3749961
TACGGGA803.6925485E-1034.37499615
TATCGGT401.8644106E-434.37499613
ATCGGTC401.8644106E-434.37499614
TGGTATC1050.034.047622
AGAGTAC3750.033.011
TATCAAC6150.032.6422772
CAGAGTA3800.032.5657910
TTGTTCG350.00404628331.4285725
TTCGCTA350.00404628331.4285728
CGCTATC350.00404628331.42857210
Produced by FastQC (version 0.11.5)