FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412138

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412138
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences136016
Sequences flagged as poor quality0
Sequence length91
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4540.3337842606752147No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2980.21909187154452417No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGT2740.20144688860134102No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2240.16468650746970945No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1520.11175155864015997No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1410.10366427479120102No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC1900.051.4429172
GTGGTAT2000.046.745961
GGTATCA4300.040.519751
GTATCAA8000.039.8403051
TATACGT559.5223026E-538.6330225
ATATCAA1401.8189894E-1236.425421
TATCAAC10100.036.184992
TCGAGGT1950.034.868789
ATCAACG10700.033.7587623
CAACGCA11050.032.689485
TCAACGC11050.032.689484
AACGCAG11700.031.599836
GTACTGA550.00443624730.9064161
AGACAGT550.00443624730.9064166
GTCGAGG2400.030.1015628
AGAGTCG7050.028.9336685
AGTCGAG2700.028.3308857
CAGAGTC7600.026.8397834
CGCAGAG13550.026.3445848
CAGAGTA7150.025.85440610-11
Produced by FastQC (version 0.11.5)