FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412139

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412139
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences136016
Sequences flagged as poor quality0
Sequence length61
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8300.610222326785084No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1970.1448359016586284No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCTAAG150.005741876655.0744061
GTCTTAT150.005741876655.0744061
TAGGTCG202.606966E-454.99337850
AGGTCGT202.606966E-454.99337851
TCGCATT303.4773562E-545.8278213
TCGTCGC303.4773562E-545.8278210
ATAAACG257.80826E-444.027093
TAAGGCT257.822433E-444.010894
CTGTGCG257.822433E-444.0108938
GGTCGTC257.8366266E-443.99470552
GTCGTCA257.8366266E-443.99470553
CTCGTCG358.6335596E-539.2809879
ACGATGC358.6335596E-539.28098725
CGTCGCA358.6335596E-539.28098711
GTGTAGG504.0457599E-738.5520821
TACTTGA300.001906956136.716272
TAATGCT300.00191733136.6757434
ACTGCGC300.001920799336.66225410
CAATTAG300.001920799336.66225446
CAGTCCA300.001920799336.6622549
Produced by FastQC (version 0.11.5)