FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412141

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412141
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences257738
Sequences flagged as poor quality0
Sequence length61
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18660.723991029650265No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5180.20097928904546478No Hit
GTATCAACGCAGAGTACGGGGTATAAGACGAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA3860.14976448952036564No Hit
GTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3500.13579681692261134No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACGTA150.00577651755.00485219
GTCTACG257.841683E-444.0038831
GTGGTAT8300.040.4252551
TGGTATC8800.038.4185142
TTAGAGG501.818274E-532.99014
AGGGCCG350.00409590631.42524552
TATACGT801.4968464E-830.9282174
ATTAGAG453.7941168E-430.5463893
CGTGCAA752.932902E-729.3302278
ATACGTG852.699744E-829.1089125
TACGTGC852.699744E-829.1089126
GAGTGGC606.303593E-527.497097
GCTATTG400.0078660527.4970878
ACGTGCA904.6946298E-827.4970877
ATAGGGC400.00787350627.4917493
GTATCAA31400.027.3272511
CAGAGTA19700.025.68754610
ACGGGAC759.722129E-625.66394816
AGAGTAC19900.025.4293811
GTGATAC651.085103E-425.3868561
Produced by FastQC (version 0.11.5)