FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412142

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412142
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55736
Sequences flagged as poor quality0
Sequence length91
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1330.23862494617482416No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT940.16865221759724414No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGT780.13994545715516005No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT630.11303286924070619No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT402.244633E-974.375011
ATCCTTA200.003207396363.7500046
GTGTGCA200.003207396363.7500049
GTTACAC250.00775759351.03
AGACATT250.00775759351.06
GTATCAA2450.048.5714261
TGGTATC651.0365329E-745.7692342
CAACGCA3050.044.5901645
TATCAAC3100.043.8709682
TCAACGC3200.042.5000044
ATCAACG3250.041.8461533
AACGCAG3600.040.138896
AGAGTAC1850.034.45945710-11
AATGGTT250.002778241434.034-35
ATATCAA500.002766375434.01
CGCAGAG4100.033.170738
ACGCAGA4500.032.1111157
GGTATCA1201.0830263E-831.8749981
GCAGAGT4300.031.6279099
TCGAGGT703.895729E-430.3571459
Produced by FastQC (version 0.11.5)