FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005412144

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412144
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33597
Sequences flagged as poor quality0
Sequence length91
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGT3220.9584189064499806No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1540.4583742596065125No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1470.43753906598803466No Hit
TCCTAGTCCTCATCGCCCTCCCATCCCTACGCATCCTTTACATAACAGAC1160.34526892282048993No Hit
GAACTATCCTGCCCGCCATCATCCTAGTCCTCATCGCCCTCCCATCCCTA960.2857397981962675No Hit
GTATGTAGGAGTTGAAGATTAGTCCGCCGTAGTCGGTGTACTCGTAGGTT940.27978688573384525No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT860.2559752358841563No Hit
CTCATGAGCTGTCCCCACATTAGGCTTAAAAACAGATGCAATTCCCGGAC860.2559752358841563No Hit
GTCCCCACATTAGGCTTAAAAACAGATGCAATTCCCGGACGTCTAAACCA820.24406941095931187No Hit
CCAATGGTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCA750.223234217340834No Hit
GTTCAGTACCATTGGTGGCCAATTGATTTGATGGTAAGGGAGGGATCGTT750.223234217340834No Hit
GTACCATTGGTGGCCAATTGATTTGATGGTAAGGGAGGGATCGTTGACCT730.21728130487841177No Hit
CCTACATACTTCCCCCATTATTCCTAGAACCAGGCGACCTGCGACTCCTT660.19644611125993394No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT650.1934696550287228No Hit
GTATCAACGCAGAGTACGGGATGGCACATGCAGCGCAAGTAGGTCTACAA650.1934696550287228No Hit
GTATCAACGCAGAGTACGGGGACCATGCCGGCCAGAACCTGTTGGATCAA620.18454028633508943No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT610.1815638301038783No Hit
GTGTACTCGTAGGTTCAGTACCATTGGTGGCCAATTGATTTGATGGTAAG610.1815638301038783No Hit
GTCTTGCACTCATGAGCTGTCCCCACATTAGGCTTAAAAACAGATGCAAT600.17858737387266718No Hit
ATATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA590.1756109176414561No Hit
GGACTAGGAAGCAGATAAGGAAAATGATTATGAGGGCGTGATCATGAAAG570.16965800517903384No Hit
GTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCCT570.16965800517903384No Hit
TCCCTACGCATCCTTTACATAACAGACGAGGTCAACGATCCCTCCCTTAC560.16668154894782272No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT550.1637050927166116No Hit
GGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCATAG540.16072863648540048No Hit
GTAAGGGAGGGATCGTTGACCTCGTCTGTTATGTAAAGGATGCGTAGGGA540.16072863648540048No Hit
CATCTCAGACGCTCAGGAAATAGAAACCGTCTGAACTATCCTGCCCGCCA540.16072863648540048No Hit
GTATCAACGCAGAGTACGGGGTATAAGACGAGCAAAAAAAAAAAAAAAAA520.15477572402297823No Hit
TCCTAGTCCTGTATGCCCTTTTCCTAACACTCACAACAAAACTAACTAAT520.15477572402297823No Hit
ATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTT490.14584635532934487Illumina PCR Primer Index 10 (95% over 22bp)
GGGGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTT480.14286989909813375No Hit
CTCGTAGGTTCAGTACCATTGGTGGCCAATTGATTTGATGGTAAGGGAGG470.13989344286692262No Hit
TTCTAGGACGATGGGCATGAAACTGTGGTTTGCTCCACAGATTTCAGAGC470.13989344286692262No Hit
ATGTAGGAGTTGAAGATTAGTCCGCCGTAGTCGGTGTACTCGTAGGTTCA470.13989344286692262No Hit
CCATCATCCTAGTCCTCATCGCCCTCCCATCCCTACGCATCCTTTACATA460.1369169866357115No Hit
CTTCTATGATAGGGGAAGTAGCGTCTTGTAGACCTACTTGCGCTGCATGT460.1369169866357115No Hit
GCATGAAACTGTGGTTTGCTCCACAGATTTCAGAGCATTGACCGTAGTAT450.13394053040450038No Hit
GTACTACTCGATTGTCAACGTCAAGGAGTCGCAGGTCGCCTGGTTCTAGG450.13394053040450038No Hit
GGATAGTTCAGACGGTTTCTATTTCCTGAGCGTCTGAGATGTTAGTATTA450.13394053040450038No Hit
GTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA440.13096407417328929No Hit
TCCTAGAACCAGGCGACCTGCGACTCCTTGACGTTGACAATCGAGTAGTA430.12798761794207816No Hit
GGGTAAATACGGGCCCTATTTCAAAGATTTTTAGGGGAATTAATTCTAGG430.12798761794207816No Hit
ACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTCT420.12501116171086704Illumina PCR Primer Index 10 (95% over 24bp)
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT410.12203470547965593No Hit
CGGGAGTACTACTCGATTGTCAACGTCAAGGAGTCGCAGGTCGCCTGGTT410.12203470547965593No Hit
CTACTCGATTGTCAACGTCAAGGAGTCGCAGGTCGCCTGGTTCTAGGAAT400.11905824924844481No Hit
TCCTTTACATAACAGACGAGGTCAACGATCCCTCCCTTACCATCAAATCA400.11905824924844481No Hit
TAACAGACGAGGTCAACGATCCCTCCCTTACCATCAAATCAATTGGCCAC400.11905824924844481No Hit
CATTAGGCTTAAAAACAGATGCAATTCCCGGACGTCTAAACCAAACCACT390.11608179301723369No Hit
TCACAAGACGTCTTGCACTCATGAGCTGTCCCCACATTAGGCTTAAAAAC390.11608179301723369No Hit
CCCTTACCATCAAATCAATTGGCCACCAATGGTACTGAACCTACGAGTAC380.11310533678602257No Hit
GTTCTAGGAATAATGGGGGAAGTATGTAGGAGTTGAAGATTAGTCCGCCG380.11310533678602257No Hit
GCTCATGAGTGCAAGACGTCTTGTGATGTAATTATTATACGAATGGGGGC380.11310533678602257No Hit
ATGTAAAGGATGCGTAGGGATGGGAGGGCGATGAGGACTAGGATGATGGC380.11310533678602257No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCC380.11310533678602257No Hit
GCTCTAGAGGGGGTAGAGGGGGTGCTATAGGGTAAATACGGGCCCTATTT360.10715242432360034No Hit
CTACACGACCGGGGGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCA360.10715242432360034No Hit
TATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT350.10417596809238922No Hit
GGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT350.10417596809238922No Hit
ATCCTGCCCGCCATCATCCTAGTCCTCATCGCCCTCCCATCCCTACGCAT350.10417596809238922No Hit
GGGTAGAGGGGGTGCTATAGGGTAAATACGGGCCCTATTTCAAAGATTTT340.10119951186117809No Hit
GGTTTAGACGTCCGGGAATTGCATCTGTTTTTAAGCCTAATGTGGGGACA340.10119951186117809No Hit
GTATACCCCCGGTCGTGTAGCGGTGAAAGTGGTTTGGTTTAGACGTCCGG340.10119951186117809No Hit
GGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCCGTACT340.10119951186117809No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)