FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412145

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412145
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33597
Sequences flagged as poor quality0
Sequence length61
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7692.2888948418013513No Hit
GTATGTAGGAGTTGAAGATTAGTCCGCCGTAGTCGGTGTACTCGTAGGTTCAGTACCATTG940.27978688573384525No Hit
GAACTATCCTGCCCGCCATCATCCTAGTCCTCATCGCCCTCCCATCCCTACGCATCCTTTA780.23216358603446738No Hit
GTATCAACGCAGAGTACGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTC730.21728130487841177No Hit
GTATCAACGCAGAGTACGGGGTATAAGACGAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA730.21728130487841177No Hit
GTACCATTGGTGGCCAATTGATTTGATGGTAAGGGAGGGATCGTTGACCTCGTCTGTTATG690.2053754799535673No Hit
GTGTACTCGTAGGTTCAGTACCATTGGTGGCCAATTGATTTGATGGTAAGGGAGGGATCGT680.20239902372235619No Hit
TCCTAGTCCTCATCGCCCTCCCATCCCTACGCATCCTTTACATAACAGACGAGGTCAACGA680.20239902372235619No Hit
GTCCCCACATTAGGCTTAAAAACAGATGCAATTCCCGGACGTCTAAACCAAACCACTTTCA670.19942256749114506No Hit
GTTCAGTACCATTGGTGGCCAATTGATTTGATGGTAAGGGAGGGATCGTTGACCTCGTCTG660.19644611125993394No Hit
GTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA630.18751674256630055No Hit
GGGGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCG560.16668154894782272No Hit
CTCATGAGCTGTCCCCACATTAGGCTTAAAAACAGATGCAATTCCCGGACGTCTAAACCAA560.16668154894782272No Hit
GGGTAAATACGGGCCCTATTTCAAAGATTTTTAGGGGAATTAATTCTAGGACGATGGGCAT530.15775218025418936No Hit
GTCTTGCACTCATGAGCTGTCCCCACATTAGGCTTAAAAACAGATGCAATTCCCGGACGTC520.15477572402297823No Hit
CCAATGGTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCCTACATA510.1517992677917671No Hit
GTTCTAGGAATAATGGGGGAAGTATGTAGGAGTTGAAGATTAGTCCGCCGTAGTCGGTGTA500.148822811560556No Hit
GGACTAGGAAGCAGATAAGGAAAATGATTATGAGGGCGTGATCATGAAAGGTGATAAGCTC500.148822811560556No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA480.14286989909813375No Hit
CATCTCAGACGCTCAGGAAATAGAAACCGTCTGAACTATCCTGCCCGCCATCATCCTAGTC460.1369169866357115No Hit
CCTACATACTTCCCCCATTATTCCTAGAACCAGGCGACCTGCGACTCCTTGACGTTGACAA460.1369169866357115No Hit
GTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCCTACATACTTCCC440.13096407417328929No Hit
GTATCAACGCAGAGTACGGGGACCATGCCGGCCAGAACCTGTTGGATCAACAACCCCAATC410.12203470547965593No Hit
CGGGAGTACTACTCGATTGTCAACGTCAAGGAGTCGCAGGTCGCCTGGTTCTAGGAATAAT400.11905824924844481No Hit
CTCGTAGGTTCAGTACCATTGGTGGCCAATTGATTTGATGGTAAGGGAGGGATCGTTGACC390.11608179301723369No Hit
GTCGTGTAGCGGTGAAAGTGGTTTGGTTTAGACGTCCGGGAATTGCATCTGTTTTTAAGCC390.11608179301723369No Hit
GTACTACTCGATTGTCAACGTCAAGGAGTCGCAGGTCGCCTGGTTCTAGGAATAATGGGGG380.11310533678602257No Hit
TCCCTACGCATCCTTTACATAACAGACGAGGTCAACGATCCCTCCCTTACCATCAAATCAA380.11310533678602257No Hit
TCCTAGTCCTGTATGCCCTTTTCCTAACACTCACAACAAAACTAACTAATACTAACATCTC360.10715242432360034No Hit
GCCGTAGTCGGTGTACTCGTAGGTTCAGTACCATTGGTGGCCAATTGATTTGATGGTAAGG360.10715242432360034No Hit
TCCTAGAACCAGGCGACCTGCGACTCCTTGACGTTGACAATCGAGTAGTACTCCCGATTGA360.10715242432360034No Hit
GGATAGTTCAGACGGTTTCTATTTCCTGAGCGTCTGAGATGTTAGTATTAGTTAGTTTTGT360.10715242432360034No Hit
TAACAGACGAGGTCAACGATCCCTCCCTTACCATCAAATCAATTGGCCACCAATGGTACTG360.10715242432360034No Hit
GGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATC360.10715242432360034No Hit
GCTCATGAGTGCAAGACGTCTTGTGATGTAATTATTATACGAATGGGGGCTTCAATCGGGA360.10715242432360034No Hit
CTACTCGATTGTCAACGTCAAGGAGTCGCAGGTCGCCTGGTTCTAGGAATAATGGGGGAAG350.10417596809238922No Hit
GGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCCGTACTCTGCGTTGATA350.10417596809238922No Hit
GGTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA350.10417596809238922No Hit
TCACAAGACGTCTTGCACTCATGAGCTGTCCCCACATTAGGCTTAAAAACAGATGCAATTC350.10417596809238922No Hit
GTTATGTAAAGGATGCGTAGGGATGGGAGGGCGATGAGGACTAGGATGATGGCGGGCAGGA340.10119951186117809No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACAAC150.00573558654.998512
CGAGATG257.74861E-443.998814
GCGAGAT257.74861E-443.998813
TACGGGT358.490419E-539.2846515
ACGGGTA300.001899387136.66567216
ACGGGAC300.001899387136.66567216
GAGATGG300.001899387136.6656725
CAGAGTA3850.033.5705210
AGAGTAC3750.032.99910711
GTATCAA5150.032.620551
AGTACGG3850.032.14198713
GAGTACG3850.032.14198712
GGCACAT350.004014704431.4746281
GTGGTAT1055.456968E-1231.4746251
ACATGCA350.004044207331.427724
GCAGCGC350.004044207331.427728
AGATGGA350.004044207331.427726
CAGCGCA350.004044207331.427729
CATGCAG350.004044207331.427725
ACGGGGG801.4126272E-830.9366616
Produced by FastQC (version 0.11.5)