FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412151

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412151
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17317
Sequences flagged as poor quality0
Sequence length61
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3101.7901484090777848No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1450.8373274816654154No Hit
GTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA880.508171161286597No Hit
GGTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA470.27140959750534155No Hit
GTATCAACGCAGAGTACGGGGTATAAGACGAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA410.2367615637812554No Hit
GTATCAACGCAGAGTACGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA400.23098689149390772No Hit
GGTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA340.19633885776982157No Hit
TATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA240.13859213489634462No Hit
GTATCAACGCAGAGTACGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA190.10971877345960616No Hit
GGTATCAACGCAGAGTACGGGGTATAAGACGAGCAAAAAAAAAAAAAAAAAAAAAAAAAAA190.10971877345960616No Hit
TATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA180.10394410117225848No Hit
ATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA180.10394410117225848No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGATGG150.00562659655.1507232
TGGTATC950.040.6373752
GTGGTAT950.040.6373751
GTCGACG453.6480106E-430.5507414
GTATCAA4450.029.7442111
GGTATCA2050.029.5930711
TATCAAC4900.027.5753612
TCGACGT400.00767011227.49566315
ACGTTTT400.00767011227.49566318
GACGTTT400.00767011227.49566317
CAGAGTA3400.026.6869710
AGAGTAC3400.026.6869711
CAACGCA5050.026.1344935
ATCAACG5100.025.9532813
TCAACGC5150.025.6270284
AACGCAG5100.025.3391426
ACGGGAA901.3164918E-624.4405916
AGTACGG3750.024.19618613
GAGTACG3750.024.19618612
TACGGGA1151.6527338E-823.90927515
Produced by FastQC (version 0.11.5)