FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412153

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412153
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences309056
Sequences flagged as poor quality0
Sequence length61
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG25410.8221810933940775No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4720.15272313108303995No Hit
GTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3410.11033599088838268No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGGGC202.6007378E-455.042271
TACGGTT150.005787974754.979945
GTGGTAT5700.040.074641
CGGAGAT358.6386106E-539.2968189
CTCGTCA358.646856E-539.29045540
GTCAGCG358.655108E-539.28409643
TCAGCGC358.655108E-539.28409644
TGGTCGT458.835563E-636.65922551
CGGTCCA300.001925366136.6592251
TGGTATC6250.036.0901952
CGAGGGT1300.035.971710
CTGGTCG401.900605E-434.36802350
AGGTCGT501.816224E-532.9986422
CGCCAGC601.7080656E-632.092434
ACGTCCC350.004092619831.43236240
CTTTCGG350.00409586431.42727747
TTCGGTC350.0040991131.42219449
TCGAGGG1600.030.9462419
CATTGCG453.7865055E-430.55924229
CGCTGGT453.7901E-430.55429648
Produced by FastQC (version 0.11.5)