FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412154

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412154
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences163540
Sequences flagged as poor quality0
Sequence length91
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5390.32958297664180014No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT4280.2617096734743794No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3220.19689372630549098No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC2900.1773266479148832No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC2810.17182340711752478No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA2530.15470221352574293No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT2520.15409074232603645No Hit
ATATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2330.14247278953161308No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA2080.1271860095389507No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1980.12107129754188578No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA1770.10823040234804941No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG1730.10578451754922343No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT1690.10333863275039745No Hit
ATATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1690.10333863275039745No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC1680.10272716155069098No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA1680.10272716155069098No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC1660.10150421915127797No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGTGT150.001028176284.995724
ATACGGG555.456968E-1269.5419541
AAGTACG800.069.059021
ATCCAAG356.5875047E-660.7112241
GTGGTAT3200.053.1223261
TGGTATC3400.049.9974822
GTCGAGG2550.046.6643188
AGTCGAG3200.042.497867
TCGAGGT2750.041.725179
TATCAAC12250.036.7736552
GTATCAA10200.035.4148861
CTAACTC601.5900304E-435.4148834
GGACTCG250.002786771233.99828716-17
ATACAAT500.002782716233.9982876
ATCAACG13300.032.9118773
CAACGCA13800.031.719425
TCAACGC13950.031.3783474
AACGCAG14150.031.2351766
GGTATCA5750.031.0419141
TAGTGTA550.004438061330.9075349
Produced by FastQC (version 0.11.5)