FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412155

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412155
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences163540
Sequences flagged as poor quality0
Sequence length61
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8670.5301455301455302No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3030.18527577351106764No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCATAA2600.1589825119236884No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGA2320.14186131833190657No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCA2120.1296318943377767No Hit
GTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2020.12351718234071177No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGTTGGAAC1800.1100648159471689No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAA1750.10700745994863643No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTGTA150.005770951555.00918649
GTCTAAC150.005777946754.992351
AACGCTC150.005777946754.9923523
GTACTAG150.005777946754.992351
GTGGTAT2800.045.1722831
TGGTATC2950.042.8753932
CGGGGTA358.642187E-539.28024717
GTCTTAT358.642187E-539.2802471
TAAGCCG458.78774E-636.6727910
TTCCCCG300.001922110836.66156815
TGTAGAA300.001922110836.66156818
GTATCAA13550.028.815181
TTTACTA400.00784731227.50459146
ACGGGGT507.011658E-427.49617616
CGCAGTT400.00785904727.49617622
GCCGTCA400.00785904727.49617613
GGTATCA6300.027.4961741
AGAGTAC8500.025.88667511
TATCAAC15150.025.7719942
GTAAGCC651.0821466E-425.3888539
Produced by FastQC (version 0.11.5)