FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412157

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412157
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences283053
Sequences flagged as poor quality0
Sequence length61
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12810.4525654206102744No Hit
GTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5050.17841181686821903No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4280.15120843092989653No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCAC150.005779343754.9994737
GTGGTAT6500.040.672491
TGGTATC6650.040.1265752
AGTTAGC300.001924934936.6598328
GCCGCGA350.004091112431.4338253
ATGAATA350.004091112431.4338253
GTTACGG350.004094653831.4282720
CCTATAG453.784676E-430.5606613
TCGAAGG453.7885996E-430.55526226
CTATCGA553.4818757E-529.99971223
ACTCATA553.4818757E-529.99971254
ATAGTAC400.007856864527.5045973
GATCATT400.007863644527.49973726
CGTCTAA705.6874487E-627.49973750
CGTCCGG606.301627E-527.49973544
ACTGAGC400.00787042927.4948778
CACACCC507.0263905E-427.4948754
TAGTACC507.0263905E-427.4948754
GTATCAA23350.026.6541251
ACGTCCG651.0861966E-425.38437343
Produced by FastQC (version 0.11.5)