FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412159

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412159
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59211
Sequences flagged as poor quality0
Sequence length61
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5240.8849706980121937No Hit
CTTCTATCTTCTCCAAATTGTTTCATCCTACCACTCCCAATTAATCTTTCCATTTTCGTCT710.11991015182989646No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCAAA150.005832466854.8184131
GTGGTAT1150.038.1345441
CAGCTTA300.00191015136.6694944
TGGTATC1050.036.6074452
GTCGACT901.3915269E-930.4546761
GTATCAA4550.027.7104071
GGGTATA400.007810815727.5021219
GAGATTG400.007810815727.5021233
AAATGCG400.007810815727.5021220
TCGACTT1004.2127795E-927.4555842
CGACTTT1057.023118E-926.1703153
GGTATCA2300.025.0257951
GACTTTT1101.1525117E-824.9807554
TATCAAC5250.024.5792852
AACAGCT752.8267168E-422.00169442
ATCAACG6100.021.1722153
AACGCAG6250.020.664086
CAACGCA6000.020.6091255
TCAACGC6050.020.43884
ACGAAGG700.004919155519.64437145
Produced by FastQC (version 0.11.5)