FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412163

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412163
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences89040
Sequences flagged as poor quality0
Sequence length61
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG24892.795372866127583No Hit
GTATCAACGCAGAGTACGGGGACCATGCCGGCCAGAACCTGTTGGATCAACAACCCCAATC2090.23472596585804134No Hit
GTATCAACGCAGAGTACGGGGTATAAGACGAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA1570.17632524707996408No Hit
GGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCCGTACTCTGCGTTGATA1350.15161725067385445No Hit
GGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAA1150.12915543575920935No Hit
GTATCAACGCAGAGTACGGGAACCCCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1000.11230907457322552No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCATG150.005765680755.0011257
TGCTTAA150.005765680755.00112519
GGGAGCA150.005765680755.0011257
AGTATGG150.005765680755.0011253
CGGGGTC150.005765680755.00112517
TCGGAGA150.005765680755.0011258
CTCGGAG150.005765680755.0011257
GTCGTCC150.005765680755.00112536
CAAGTAT150.00577852554.970231
GCGTATA150.00577852554.970231
GTGGTAT3400.042.8444371
TGGTATC3550.041.034112
GGGAGCT358.6023334E-539.28651419
TATTACG358.6023334E-539.2865144
GAATAGC300.00191590736.6674253
CTGTATT300.00192120636.646821
AGATCGA350.004079107731.4292136
AGCGCCG350.004090355231.4115562
GTATCAA17350.030.7326341
AAGAGAC453.7672705E-430.5561817
Produced by FastQC (version 0.11.5)