FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412164

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412164
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29825
Sequences flagged as poor quality0
Sequence length91
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3881.3009220452640402No Hit
GGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAA3781.267393126571668No Hit
ATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAA3131.049455155071249No Hit
AACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAAA2820.9455155071248953No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2460.8248113998323554No Hit
CAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAA2210.740989103101425No Hit
GTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1960.6571668063704945No Hit
CCGCGAGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGT1950.6538139145012574No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1940.6504610226320201No Hit
AGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAA1850.6202849958088852No Hit
GGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAA1420.4761106454316848No Hit
GATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAA1420.4761106454316848No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1350.45264040234702435No Hit
GAGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCA1240.41575859178541497No Hit
CTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCGGGGTTGGCGG1230.4124056999161777No Hit
GGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1170.3922883487007544No Hit
CTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGT1070.35875943000838223No Hit
TTTTTGGGACCATGCCGGCCAGAACCTGTTGGATCAACAACCCCAATCCA1050.3520536462699078No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1010.33864207879295893No Hit
GAGCGCCGCGAGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGC1000.3352891869237217No Hit
GGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATCTGTCTCTTATA990.33193629505448446No Hit
TGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAA950.3185247275775356No Hit
GATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAA940.31517183570829843No Hit
ATGCCGGCCAGAACCTGTTGGATCAACAACCCCAATCCCTGTCTCTTATA940.31517183570829843No Hit
GTATCAACGCAGAGTACGGGGACCATGCCGGCCAGAACCTGTTGGATCAA910.30511316010058676No Hit
GGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT900.30176026823134955No Hit
GTATCAACGCAGAGTACGGGACCATGCCGGCCAGAACCTGTTGGATCAAC860.28834870075440067No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT840.2816429170159262No Hit
GGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAC840.2816429170159262No Hit
CTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCGCGTATGCCGT820.2749371332774518No Hit
TTTTGGGACCATGCCGGCCAGAACCTGTTGGATCAACAACCCCAATCCAT810.2715842414082146No Hit
CTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCGGGGATGGCGG800.26823134953897737No Hit
TTTTTGGGACCATGCCGGCCAGAACCTGTTGGATCAACAACCCCAATCCC780.2615255658005029No Hit
TATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT760.2548197820620285No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG730.24476110645431687No Hit
GCGAGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCC710.2380553227158424No Hit
CTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCGCGTATGCCGG710.2380553227158424No Hit
GGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAACT700.2347024308466052No Hit
ATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAA640.2145850796311819No Hit
TTTTGGGACCATGCCGGCCAGAACCTGTTGGATCAACAACCCCAATCCCT640.2145850796311819No Hit
GGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAA630.21123218776194466No Hit
GCGCCGCGAGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCAT620.20787929589270748No Hit
GGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCCGTACT620.20787929589270748No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT580.19446772841575857No Hit
GATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAA580.19446772841575857No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG570.19111483654652137No Hit
CTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCGGGTATGGCGG560.18776194467728416No Hit
CTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCGGGGGTGGCGG540.18105616093880972No Hit
ATTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA530.1777032690695725No Hit
GGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCTGTCTC520.1743503772003353No Hit
GGCCAGAACCTGTTGGATCAACAACCCCAATCCATCTCGCGGCTGTCTCT510.17099748533109807No Hit
CTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCGCGGATGCCGG510.17099748533109807No Hit
GGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAACTGTCTC510.17099748533109807No Hit
TCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAA500.16764459346186086No Hit
CTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCGGGTTTGGCGG490.16429170159262363No Hit
CTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGG490.16429170159262363No Hit
TGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAA480.16093880972338642No Hit
GTGTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGACTACGCTG480.16093880972338642No Hit
ATGCCGGCCAGAACCTGTTGGATCAACAACCCCAATCCATCTCTGTCTCT470.15758591785414922No Hit
GTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAA460.15423302598491198No Hit
CTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCGCGTATGGCGG450.15088013411567477No Hit
GGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCGTACTC450.15088013411567477No Hit
GGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAACTGTCTCT440.14752724224643754No Hit
CTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCGGGGTTTGCGG440.14752724224643754No Hit
CCGCGAGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCCTGTCTCT440.14752724224643754No Hit
GTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAA440.14752724224643754No Hit
TTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAA440.14752724224643754No Hit
ATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT440.14752724224643754No Hit
GGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT420.1408214585079631No Hit
GTATCAACGCAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT420.1408214585079631No Hit
CTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCGGGGATGCCGG410.1374685666387259No Hit
CTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCGCGGATGGCGG400.13411567476948869No Hit
GGACCATGCCGGCCAGAACCTGTTGGATCAACAACCCCAATCCCTGTCTC390.13076278290025145No Hit
GAGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATCTGTCTC390.13076278290025145No Hit
TTTTTGGGACCATGCCGGCCAGAACCTGTTGGATCAACAACCCCTGTCTC390.13076278290025145No Hit
ATGCCGGCCAGAACCTGTTGGATCAACAACCCCAATCCATCTCGCGGCTG380.12740989103101424No Hit
CGAGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCC370.12405699916177702No Hit
GATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATCTGTCTCTTATAC370.12405699916177702No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG370.12405699916177702No Hit
GGCCAGAACCTGTTGGATCAACAACCCCAATCCATCTCTGTCTCTTATAC370.12405699916177702No Hit
GTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGACTACGCTGCA370.12405699916177702No Hit
AGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCCTGTCTCTTATAC360.12070410729253982No Hit
CTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCGCGGATGCCGT360.12070410729253982No Hit
CTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGGCGT360.12070410729253982No Hit
AGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATCTGTCTCT350.1173512154233026No Hit
CTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCGCGTATGGCGT350.1173512154233026No Hit
CTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCGCGGTTGGCGG350.1173512154233026No Hit
ATGCCGGCCAGAACCTGTTGGATCAACAACCCCAATCCATCTCCTGTCTC350.1173512154233026No Hit
GGACCATGCCGGCCAGAACCTGTTGGATCAACAACCCCAATCCATCTCGC340.11399832355406539No Hit
GGCCAGAACCTGTTGGATCAACAACCCCAATCCCTGTCTCTTATACACAT340.11399832355406539No Hit
CTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCGGGGTTGCCGG340.11399832355406539No Hit
TTTTTGGGACCATGCCGGCCAGAACCTGTTGGATCAACAACCCCAATCCT340.11399832355406539No Hit
TATCAACGCAGAGTACGGGGACCATGCCGGCCAGAACCTGTTGGATCAAC330.11064543168482815No Hit
CTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCGGGTATGCCGG330.11064543168482815No Hit
CTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCGCGTTTGGCGG320.10729253981559095No Hit
TATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT320.10729253981559095No Hit
GGATTGGGGTTGTTGATCCAACAGGTTCTGGCCTGTCTCTTATACACATC310.10393964794635374No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG300.10058675607711651No Hit
GATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCTGTCTCT300.10058675607711651No Hit
ATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG300.10058675607711651No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCAGA202.9864326E-584.964761
AGATGTG259.025763E-567.971815
CAGATGT259.025763E-567.971814
GACAGAT200.003197995263.723579
GTGGTAT454.2845204E-756.6431771
TCAGATG302.2241929E-456.6431733
GTCAGAT302.2241929E-456.6431732
TGGTATC508.8673005E-750.978862
GATGTGT354.7609067E-448.5512966
AGCGCCG452.865132E-547.202652
GGTATCA1200.046.022581
GTATCAA2650.044.8870471
GCGTGGA209.190819E-442.5537856-57
GAGCGCC505.337778E-542.4823841
TGGGCGT255.4029722E-542.48238452-53
GGGCGTG351.8649189E-742.48238454-55
TCGTGTG209.2672266E-442.4823850-51
ATGTGTA409.1925083E-442.482387
GCCGGGT209.2672266E-442.4823846-47
TGTGTAT409.1925083E-442.482388
Produced by FastQC (version 0.11.5)