FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005412165

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412165
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29825
Sequences flagged as poor quality0
Sequence length61
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG499516.747694886839902No Hit
GGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAA2930.9823973176865046No Hit
ATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1870.6269907795473596No Hit
AACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1410.47275775356244765No Hit
GTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1380.46269907795473597No Hit
AGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAA1160.38893545683151715No Hit
GGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAA1130.3788767812238055No Hit
CAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1010.33864207879295893No Hit
GATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAA950.3185247275775356No Hit
GGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATCTGTCTCTTATACACATCTGACG880.2950544844928751No Hit
ATGCCGGCCAGAACCTGTTGGATCAACAACCCCAATCCCTGTCTCTTATACACATCTGACG880.2950544844928751No Hit
GGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA850.28499580888516346No Hit
GGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAACTGTCTCTTATA810.2715842414082146No Hit
TTTTTGGGACCATGCCGGCCAGAACCTGTTGGATCAACAACCCCAATCCCTGTCTCTTATA770.2581726739312657No Hit
GATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA760.2548197820620285No Hit
GGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAA680.22799664710813078No Hit
TTTTGGGACCATGCCGGCCAGAACCTGTTGGATCAACAACCCCAATCCCTGTCTCTTATAC660.2212908633696563No Hit
GTATCAACGCAGAGTACGGGGACCATGCCGGCCAGAACCTGTTGGATCAACAACCCCAATC640.2145850796311819No Hit
GGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAACTGTCTCTTATAC640.2145850796311819No Hit
TGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA630.21123218776194466No Hit
GGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA580.19446772841575857No Hit
GTATCAACGCAGAGTACGGGACCATGCCGGCCAGAACCTGTTGGATCAACAACCCCAATCC570.19111483654652137No Hit
GTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA550.18440905280804692No Hit
GAGCGCCGCGAGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAA530.1777032690695725No Hit
CCGCGAGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAA510.17099748533109807No Hit
GAGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAA490.16429170159262363No Hit
TTTTTGGGACCATGCCGGCCAGAACCTGTTGGATCAACAACCCCTGTCTCTTATACACATC480.16093880972338642No Hit
CCGCGAGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCCTGTCTCTTATACACATCT470.15758591785414922No Hit
GTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAA450.15088013411567477No Hit
GGACCATGCCGGCCAGAACCTGTTGGATCAACAACCCCAATCCCTGTCTCTTATACACATC440.14752724224643754No Hit
AGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATCTGTCTCTTATACACATCT440.14752724224643754No Hit
GGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCCGTACTCTGCGTTGATA410.1374685666387259No Hit
TTTTGGGACCATGCCGGCCAGAACCTGTTGGATCAACAACCCCTGTCTCTTATACACATCT410.1374685666387259No Hit
GGCCAGAACCTGTTGGATCAACAACCCCAATCCATCTCGCGGCTGTCTCTTATACACATCT390.13076278290025145No Hit
GTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAAA390.13076278290025145No Hit
GGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCTGTCTCTTATACACATC380.12740989103101424No Hit
AGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCCTGTCTCTTATACACATCTGACGC380.12740989103101424No Hit
GGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAACTGTCTCTTATACACATC360.12070410729253982No Hit
TCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA360.12070410729253982No Hit
ATGCCGGCCAGAACCTGTTGGATCAACAACCCCAATCCTGTCTCTTATACACATCTGACGC360.12070410729253982No Hit
CCGCGAGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATCTGTCTCTTATACA350.1173512154233026No Hit
GGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCGTACTCTGCGTTGATAC350.1173512154233026No Hit
ATGCCGGCCAGAACCTGTTGGATCAACAACCCCAATCCATCTCCTGTCTCTTATACACATC340.11399832355406539No Hit
GAGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATCTGTCTCTTATACACATC340.11399832355406539No Hit
GATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAACTGTCTCTTATAC340.11399832355406539No Hit
GCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA330.11064543168482815No Hit
GGATTGGGGTTGTTGATCCAACAGGTTCTGGCCCTGTCTCTTATACACATCTGACGCTGCC320.10729253981559095No Hit
TGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA320.10729253981559095No Hit
GGCCAGAACCTGTTGGATCAACAACCCCAATCCATCTCTGTCTCTTATACACATCTGACGC310.10393964794635374No Hit
TTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAAAA310.10393964794635374No Hit
GCCAGAACCTGTTGGATCAACAACCCCAATCCCTGTCTCTTATACACATCTGACGCTGCCG300.10058675607711651No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)