FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005412166

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412166
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49976
Sequences flagged as poor quality0
Sequence length91
%GC44

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3810.7623659356491116No Hit
ATATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3360.6723227149031535No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2630.5262526012485993No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1960.39218825036017285No Hit
TATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1610.32215463422442775No Hit
ATATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1590.31815271330238515No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1490.29814310869217225No Hit
GGTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA1460.2921402273091084No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1440.2881383063870658No Hit
GCTTATAAACAACGGGAAGGAACTGAGGAACTTTTGCAAATTATCTCCCC1260.2521210180886826No Hit
AGTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA1160.2321114134784697No Hit
GGGCAAAGACCATGTGAGGAAACAGAGAAGGTGGCTGTCTGCAAGCCAGG1140.2281094925564271No Hit
CCCCAGAAGCGTTGGCTCATCAGAGCCAAACTCAGATGTTAACTGGAATG1100.2201056507123419No Hit
GTATCAACGCAGAGTACGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA1050.21010084840723547No Hit
GTTTAAGGCCACACCATGAGAAAAGGCAGAGCTGAGGCTTGGGCTGGGTC920.1840883624139587No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT910.1820874019529374No Hit
GTGTAAGCTAGTAACATCCTTATTTACTAAGCTGCTTATAAACAACGGGA910.1820874019529374No Hit
GTATCAACGCAGAGTACGGGGACCATGCCGGCCAGAACCTGTTGGATCAA860.17208259964783096No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT780.15607491595966064No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT780.15607491595966064No Hit
TATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA770.15407395549863934No Hit
ATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA760.15207299503761804No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT750.15007203457659676No Hit
GTCATAGATTCTCACAGGTGAAAGAGACTTTGGAGAATCTCTGGACTAAC740.14807107411557546No Hit
TCTCTGGACTAACTCATTTCCCAGATGAGAAAACTGGAGCCAGAGAAGGG720.1440691531935329No Hit
ATATCAACGCAGAGTACGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA700.14006723227149032No Hit
GTCCAAGATCAAGGTGTCAGCAGATTCAGTTTCTGGTGAGGGCTCTCTTC680.13606531134944774No Hit
GGTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA640.12806146950536257No Hit
GTGTAGACCCAGCCCAAGCCTCAGCTCTGCCTTTTCTCATGGTGTGGCCT630.1260605090443413No Hit
CACTTTGATCAGTGTCCTTCTCTAACCTCCATCCCTCACCTCCCTGTCAA600.12005762766127742No Hit
GGTCTACACACACTTTGATCAGTGTCCTTCTCTAACCTCCATCCCTCACC580.11605570673923485No Hit
AGTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA560.11205378581719225No Hit
ATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT560.11205378581719225No Hit
ACCATGCCCAGTGGACACTGGGCTCTGGGAAATGAAGCTGGGGTGGATTG550.11005282535617095No Hit
GTTGAAGGTTGGTCAACAGAGGTAGTGAAAGCACTTGGGCCTCCAGGGCC550.11005282535617095No Hit
GTATGGAATGTCCAAATTGAAATTATGCCTCTTATTTAGAGAGTCATAGA550.11005282535617095No Hit
GTGAATGTTCCTTCCATGCTCATTTCTGTTGAAGGTTGGTCAACAGAGGT540.10805186489514966No Hit
CTTCTGGGGAGATAATTTGCAAAAGTTCCTCAGTTCCTTCCCGTTGTTTA530.10605090443412837No Hit
GGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT520.10404994397310709No Hit
CTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGT520.10404994397310709No Hit
TATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT510.1020489835120858No Hit
TATGTACATAACGTGATTAGACAAGCTGATGTTTTTCAAAGGCCAGTCAT510.1020489835120858No Hit
TCTCTAACCTCCATCCCTCACCTCCCTGTCAATCCACCCCAGCTTCATTT510.1020489835120858No Hit
GCCTTAAACAAGTCACTTCCCTTCTCTGGCTCCAGTTTTCTCATCTGGGA510.1020489835120858No Hit
GGTATCAACGCAGAGTACGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA500.1000480230510645No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)