FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412167

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412167
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49976
Sequences flagged as poor quality0
Sequence length61
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18053.611733632143429No Hit
GTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4730.9464542980630702No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2670.5342564430926845No Hit
GGTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1780.35617096206178966No Hit
TATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1100.2201056507123419No Hit
GTATCAACGCAGAGTACGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1060.21210180886825675No Hit
GGGCAAAGACCATGTGAGGAAACAGAGAAGGTGGCTGTCTGCAAGCCAGGAAGAGAGCCCT950.19009124379702258No Hit
GCTTATAAACAACGGGAAGGAACTGAGGAACTTTTGCAAATTATCTCCCCAGAAGCGTTGG830.16607971826476708No Hit
GGTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA810.1620777973427245No Hit
CCCCAGAAGCGTTGGCTCATCAGAGCCAAACTCAGATGTTAACTGGAATGAGCTACTAACT800.16007683688170324No Hit
GTGTAAGCTAGTAACATCCTTATTTACTAAGCTGCTTATAAACAACGGGAAGGAACTGAGG800.16007683688170324No Hit
GTTTAAGGCCACACCATGAGAAAAGGCAGAGCTGAGGCTTGGGCTGGGTCTACACACACTT760.15207299503761804No Hit
ATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA680.13606531134944774No Hit
GTATCAACGCAGAGTACGGGGACCATGCCGGCCAGAACCTGTTGGATCAACAACCCCAATC630.1260605090443413No Hit
GTCCAAGATCAAGGTGTCAGCAGATTCAGTTTCTGGTGAGGGCTCTCTTCCTGGCTTGCAG580.11605570673923485No Hit
TATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA580.11605570673923485No Hit
TCTCTGGACTAACTCATTTCCCAGATGAGAAAACTGGAGCCAGAGAAGGGAAGTGACTTGT570.11405474627821355No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTTTGATCACGTTT560.11205378581719225No Hit
GTATGGAATGTCCAAATTGAAATTATGCCTCTTATTTAGAGAGTCATAGATTCTCACAGGT550.11005282535617095No Hit
GTCATAGATTCTCACAGGTGAAAGAGACTTTGGAGAATCTCTGGACTAACTCATTTCCCAG540.10805186489514966No Hit
GTTGAAGGTTGGTCAACAGAGGTAGTGAAAGCACTTGGGCCTCCAGGGCCACCATGCCCAG530.10605090443412837No Hit
CACTTTGATCAGTGTCCTTCTCTAACCTCCATCCCTCACCTCCCTGTCAATCCACCCCAGC520.10404994397310709No Hit
GTGAATGTTCCTTCCATGCTCATTTCTGTTGAAGGTTGGTCAACAGAGGTAGTGAAAGCAC510.1020489835120858No Hit
GGTCTACACACACTTTGATCAGTGTCCTTCTCTAACCTCCATCCCTCACCTCCCTGTCAAT510.1020489835120858No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCATTC150.00575968554.9799771
CATTCGC150.00575968554.9799773
ATTCGCC150.00575968554.9799774
GTGGTAT1250.050.581581
TAAGCTA351.5455789E-647.1256984
GTGTAAG351.5455789E-647.1256981
GTAAGCT351.5455789E-647.1256983
AAGCTAG351.5455789E-647.1256985
CTCAGAG257.798261E-443.9839868
TGGTATC1400.043.198562
GCTAGTA403.874213E-641.2349857
TGTAAGC403.874213E-641.2349852
TAAGAGA358.567538E-539.2714164
AAGCTGC504.0315535E-738.4859929
ACTAAGC458.697643E-636.6533226
TTACTAA458.697643E-636.6533224
CAACTCA300.001911478936.653325
CTAAGCT458.697643E-636.6533227
TGCTTAT458.697643E-636.6533233
TCAGAGT300.001911478936.653329
Produced by FastQC (version 0.11.5)