FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412169

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412169
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences957188
Sequences flagged as poor quality0
Sequence length61
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG42830.4474565080214127No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11060.11554678913651237No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9650.10081614061187563No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGCT150.005784642454.99681551
GTGGTAT22500.043.0647351
TGGTATC24050.040.4885182
TTGCGTT358.664257E-539.287544
GCTTACT300.001923047136.676042
TACGGCA751.9826984E-1036.66837338
CCCGTTA300.00192551636.6664643
TGCGCGT658.391544E-833.8459612
TAAGCCG758.001734E-933.00153436
GCATCGT1300.031.7305878
CGGCGAT701.6092235E-731.4267532
CCGTCCG801.5015758E-830.93732354
CTTGCTC1350.030.5585753
CGACGAG1350.030.55378524
CTGGTCG1300.029.61366850
CAATGCG1400.029.46257819
CGGTCGC752.9457806E-729.33163553
ACGCCTA752.9471266E-729.3301016
CGTAACG1052.3101165E-1028.80785623
CCGTGTT705.705877E-627.4998449
Produced by FastQC (version 0.11.5)