FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412170

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412170
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences933277
Sequences flagged as poor quality0
Sequence length91
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18980.203369417654137No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT17930.19211873859529377No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12870.1379011804641066No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTACG2650.049.730931
GTGGTAT11400.044.7493931
TGGTATC14550.037.6829072
GGTATCA22100.037.318161
GTATCAA39400.033.8800621
TACCGAC550.004449641330.9061687
TATCAAC47350.030.6989672
ATCAACG49200.029.5446383
CAACGCA50500.028.6983815
TCAACGC50500.028.6983814
CGTTACA600.00680273828.335212
CCGACCG600.0068080828.3306569
ACATCGG600.0068080828.3306568
AACGCAG52600.027.5526286
GTCGAGG8700.027.3537358
TCGAGGT8750.026.7117619
TATACCG850.001226291925.0003155
AGTCGAG11050.024.6130587
TATACGT1401.5308215E-624.286024
ACGCAGA60800.023.903997
Produced by FastQC (version 0.11.5)