FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412171

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412171
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences933277
Sequences flagged as poor quality0
Sequence length61
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG28030.3003395562089283No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT11400.035.489161
TACCGAG350.00410348731.4219287
TGGTATC13400.029.7639312
ATAACCG400.007880514527.4941845
GGTATCA23050.025.9102761
TCCGAGA651.08912325E-425.3833323
TATACGG550.001224828525.0054342
GTATCAA42100.024.4496571
ATATCGG852.5685282E-522.6483443
TATCAAC47600.021.6696262
AGAGTAC26900.021.56838411
CAGAGTA28150.020.90368310
CATATCG956.0554026E-520.26430342
TCGATGG956.0604E-520.2621319
CTTATCG956.0604E-520.2621318
GTCCTAA1251.1142147E-619.8159891
CGAGGGT3900.019.74153310
ATCAACG52050.019.652133
TTCGGTG700.00497680419.64186117
TACGTTT700.004981433519.6387046
Produced by FastQC (version 0.11.5)