FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412173

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412173
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences140494
Sequences flagged as poor quality0
Sequence length61
%GC42

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG49233.5040642305009464No Hit
GGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAA23611.6804988113371389No Hit
ATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14030.9986191581135136No Hit
GGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAA10320.7345509416772246No Hit
GATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAA9970.7096388457870086No Hit
AACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9530.6783207823821658No Hit
GTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7880.5608780446140048No Hit
AGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAA7420.5281364328725782No Hit
CAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6790.48329466027018947No Hit
GGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAA5680.4042877275897903No Hit
GGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATCTGTCTCTTATACACATCTGACG5020.3573106324825259No Hit
CCGCGAGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAA4800.3416516007801045No Hit
GATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4720.3359574074337694No Hit
ATGCCGGCCAGAACCTGTTGGATCAACAACCCCAATCCCTGTCTCTTATACACATCTGACG4670.33239853659231No Hit
GGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4290.3053511181972184No Hit
TGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA4190.29823337651429954No Hit
GAGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAA3850.27403305479237544No Hit
GAGCGCCGCGAGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAA3770.2683388614460404No Hit
GGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3510.2498327330704514No Hit
GGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCTGTCTCTTATACACATC3290.23417370136802995No Hit
TTTTTGGGACCATGCCGGCCAGAACCTGTTGGATCAACAACCCCAATCCCTGTCTCTTATA3270.23275015303144617No Hit
GTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAA3200.227767733853403No Hit
GGACCATGCCGGCCAGAACCTGTTGGATCAACAACCCCAATCCCTGTCTCTTATACACATC3120.2220735405070679No Hit
GGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAACTGTCTCTTATA3100.22064999217048414No Hit
GTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAAA3050.21709112132902472No Hit
TTTTGGGACCATGCCGGCCAGAACCTGTTGGATCAACAACCCCAATCCCTGTCTCTTATAC2970.21139692798268966No Hit
GGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAACTGTCTCTTATAC2720.19360257377539256No Hit
AGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATCTGTCTCTTATACACATCT2640.18790838042905747No Hit
CCGCGAGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCCTGTCTCTTATACACATCT2600.18506128375588993No Hit
GGCCAGAACCTGTTGGATCAACAACCCCAATCCATCTCGCGGCTGTCTCTTATACACATCT2560.18221418708272238No Hit
GTATCAACGCAGAGTACGGGGACCATGCCGGCCAGAACCTGTTGGATCAACAACCCCAATC2430.1729611228949279No Hit
ATGCCGGCCAGAACCTGTTGGATCAACAACCCCAATCCATCTCTGTCTCTTATACACATCT2310.1644198328754253No Hit
TCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2270.16157273620225773No Hit
TTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAAAA2240.1594374136973821No Hit
ATGCCGGCCAGAACCTGTTGGATCAACAACCCCAATCCTGTCTCTTATACACATCTGACGC2220.15801386536079834No Hit
GATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAA2200.15659031702421455No Hit
ATGCCGGCCAGAACCTGTTGGATCAACAACCCCAATCCATCTCCTGTCTCTTATACACATC2200.15659031702421455No Hit
GCGCCGCGAGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAA2160.153743220351047No Hit
CCGCGAGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATCTGTCTCTTATACA2140.15231967201446325No Hit
TGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2060.14662547866812817No Hit
GGATTGGGGTTGTTGATCCAACAGGTTCTGGCCCTGTCTCTTATACACATCTGACGCTGCC2040.1452019303315444No Hit
GAGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATCTGTCTCTTATACACATC2020.14377838199496065No Hit
ATGCCGGCCAGAACCTGTTGGATCAACAACCCCAATCCATCTCGCGGCTGTCTCTTATACA2020.14377838199496065No Hit
GGCCAGAACCTGTTGGATCAACAACCCCAATCCCTGTCTCTTATACACATCTGACGCTGCC2020.14377838199496065No Hit
GTATCAACGCAGAGTACGGGACCATGCCGGCCAGAACCTGTTGGATCAACAACCCCAATCC1990.141643059490085No Hit
ATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAA1950.13879596281691745No Hit
GCGAGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAA1840.13096644696570672No Hit
GATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATCTGTCTCTTATACACATCTGACGC1830.13025467279741484No Hit
GGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAGCAAAAAAAAAAAAAA1780.12669580195595542No Hit
TTTTTGGGACCATGCCGGCCAGAACCTGTTGGATCAACAACCCCTGTCTCTTATACACATC1660.11815451193645281No Hit
GGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAACTGTCTCTTATACACATC1610.11459564109499339No Hit
AGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCCTGTCTCTTATACACATCTGACGC1560.11103677025353395No Hit
TTTTTGGGACCATGCCGGCCAGAACCTGTTGGATCAACAACCCCAATCCTGTCTCTTATAC1550.11032499608524207No Hit
GGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAACTGTCTCTTATACACAT1510.10747789941207453No Hit
GGACCATGCCGGCCAGAACCTGTTGGATCAACAACCCCAATCCTGTCTCTTATACACATCT1490.10605435107549077No Hit
GATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAACTGTCTCTTATAC1460.10391902857061512No Hit
GGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCCGTACTCTGCGTTGATA1450.10320725440232323No Hit
AGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCTGTCTCTTATACA1450.10320725440232323No Hit
GTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1430.10178370606573947No Hit
GGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAACTGTCTCTTATACACATCT1410.1003601577291557No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT7850.049.040141
TGGTATC7900.048.7124142
GTGTATA508.229108E-943.996011
AGATTGG257.8363606E-443.996011
TGTATAA508.256393E-943.9803472
CAGTGGT451.7905222E-742.77391
ACGAGCA403.9272036E-641.2315758
ATAAGAC358.651641E-539.268173
TAAGACG358.651641E-539.268174
AAGACGA358.651641E-539.268175
AGACGAG358.651641E-539.268176
AGTGGTA504.1045496E-738.4828032
AAGAAGG300.001917375536.67640355
GAATTGG300.001920733736.663341
GACGAGC401.8980594E-434.3596467
AGCGCCG2700.033.59612
CGAGCAA501.8098188E-532.997019
GATGGGG501.8142375E-532.985262
GAGCGCC2700.032.5896381
GGATGGG350.004089312631.4257241
Produced by FastQC (version 0.11.5)