FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005412174

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412174
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences116818
Sequences flagged as poor quality0
Sequence length91
%GC43

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGT24292.0793028471639645No Hit
GGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAA16311.396188943484737No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13841.1847489256792618No Hit
ATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAA12351.0572000890273758No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT10590.9065383759352154No Hit
AACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAAA9580.8200790973993735No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8680.7430361759317913No Hit
CCGCGAGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGT8040.688250098443733No Hit
CAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAA6790.581246040849869No Hit
AGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAA6700.5735417487031108No Hit
GATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAA6450.552140937184338No Hit
GTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6410.5487168073413343No Hit
GGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAA6100.5221798010580562No Hit
ATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTT5980.5119074115290452RNA PCR Primer, Index 47 (95% over 22bp)
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5310.4545532366587341No Hit
GAGCGCCGCGAGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGC5200.44513687959047404No Hit
GAGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCA4430.37922238011265386No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG3970.33984488691811193No Hit
GGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3790.3244363026245955No Hit
GTATCAACGCAGAGTACGGGGACCATGCCGGCCAGAACCTGTTGGATCAA3730.31930010786009005No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT3640.31159581571333184No Hit
GATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAA3390.29019500419455907No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3310.28334674450855174No Hit
ACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCT3190.27307435497954086RNA PCR Primer, Index 47 (95% over 24bp)
TATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3140.2687941926757863No Hit
GGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3080.26365799791128075No Hit
GGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAA3030.25937783560752625No Hit
TGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAA2990.2559537057645226No Hit
GCGCCGCGAGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCAT2850.24396925131400984No Hit
GGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATCTGTCTCTTATA2780.23797702408875346No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT2700.23112876440274616No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG2630.22513653717748977No Hit
TTTTTGGGACCATGCCGGCCAGAACCTGTTGGATCAACAACCCCAATCCA2620.22428050471673885No Hit
TTTTGGGACCATGCCGGCCAGAACCTGTTGGATCAACAACCCCAATCCAT2590.22171240733448613No Hit
ATGCCGGCCAGAACCTGTTGGATCAACAACCCCAATCCCTGTCTCTTATA2550.21828827749148247No Hit
GCGAGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCC2480.21229605026622608No Hit
ATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAA2160.18490301152219693No Hit
GTATCAACGCAGAGTACGGGACCATGCCGGCCAGAACCTGTTGGATCAAC2030.17377458953243508No Hit
GTAATAGACTAGATGGCCCTTGGGACTGATCCAAGTGTGACATTTCTAAG1960.1677823623071787No Hit
TCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAA1960.1677823623071787No Hit
GGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCCGTACT1950.16692632984642777No Hit
ATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1920.16435823246417502No Hit
GTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAA1760.15066171309216045No Hit
GTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAA1720.1472375832491568No Hit
GGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAC1660.14210138848465134No Hit
CCGCGAGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCCTGTCTCT1630.1395332911023986No Hit
GTATCAACGCAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1630.1395332911023986No Hit
GATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAA1630.1395332911023986No Hit
GGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1620.13867725864164768No Hit
TTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAA1610.13782122618089676No Hit
CGAGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCC1610.13782122618089676No Hit
TCCAGGAACCATGCAGGATGGCAGCTCTCCACGTGTCTTAGGATGGGGAG1590.13610916125939496No Hit
GGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAGCAAA1590.13610916125939496No Hit
TGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAA1590.13610916125939496No Hit
GGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCTGTCTC1580.13525312879864404No Hit
GTCTTGGCTCACTGCAACCTCCACCTCCTGGGTTCAAGCGATTCTCCTGC1560.13354106387714224No Hit
GGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCAAAAAAAAAAAAAA1540.13182899895564038No Hit
GGCCAGAACCTGTTGGATCAACAACCCCAATCCATCTCGCGGCTGTCTCT1510.12926090157338765No Hit
TTTTGGGACCATGCCGGCCAGAACCTGTTGGATCAACAACCCCAATCCCT1500.12840486911263677No Hit
TTTTTGGGACCATGCCGGCCAGAACCTGTTGGATCAACAACCCCAATCCC1470.12583677173038402No Hit
AGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAG1460.12498073926963311No Hit
ATTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1450.12412470680888218No Hit
AGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATCTGTCTCT1430.12241264188738038No Hit
GGTATCAACGCAGAGTACGGGGACCATGCCGGCCAGAACCTGTTGGATCA1420.12155660942662946No Hit
GAGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATCTGTCTC1420.12155660942662946No Hit
GTGGTATCAACGCAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTT1420.12155660942662946No Hit
TATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1400.11984454450512762No Hit
GTTCCTGGATGTGAGACAAATGTTACTGTACATTTTTCTAAAGAAGAAAC1360.116420414662124No Hit
GGACCATGCCGGCCAGAACCTGTTGGATCAACAACCCCAATCCATCTCGC1340.11470834974062216No Hit
TATCAACGCAGAGTACGGGGACCATGCCGGCCAGAACCTGTTGGATCAAC1310.11214025235836943No Hit
GGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCGTACTC1300.11128421989761851No Hit
GGACCATGCCGGCCAGAACCTGTTGGATCAACAACCCCAATCCCTGTCTC1290.11042818743686761No Hit
GGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAACT1270.10871612251536578No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG1260.10786009005461489No Hit
CTCTAATGTTACGCAATATTCCTATGGTTACAGAGCCCGAATAAGTGGAA1240.10614802513311304No Hit
ATGCCGGCCAGAACCTGTTGGATCAACAACCCCAATCCATCTCTGTCTCT1220.10443596021161124No Hit
ATGCCGGCCAGAACCTGTTGGATCAACAACCCCAATCCATCTCCTGTCTC1220.10443596021161124No Hit
TTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTC1170.10015579790785667No Hit
ATGCCGGCCAGAACCTGTTGGATCAACAACCCCAATCCATCTCGCGGCTG1170.10015579790785667No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG1170.10015579790785667No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)