FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412176

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412176
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences229510
Sequences flagged as poor quality0
Sequence length91
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGT38331.6700797350877958No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34801.5162738007058516No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT23731.0339418761709729No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19510.8500718922922748No Hit
GGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAA15060.6561805585813254No Hit
GTATCAACGCAGAGTACGGGGACCATGCCGGCCAGAACCTGTTGGATCAA13420.5847239771687508No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12030.5241601673129711No Hit
ATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAA10420.4540107184872119No Hit
CCGCGAGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGT10110.44050368175678617No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9590.41784671691865277No Hit
AACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAAA8850.3856041131105398No Hit
ATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTT8200.3572829070628731RNA PCR Primer, Index 47 (95% over 22bp)
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT8200.3572829070628731No Hit
TATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7750.3376759182606422No Hit
GGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCCGTACT7210.3141475316979653No Hit
CAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAA6960.3032547601411703No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT6960.3032547601411703No Hit
AGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAA6750.2941048320334626No Hit
GAGCGCCGCGAGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGC6330.27580497581804714No Hit
GGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6280.2736264215066882No Hit
GTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6170.26883360202169837No Hit
GTATCAACGCAGAGTACGGGACCATGCCGGCCAGAACCTGTTGGATCAAC6100.26578362598579586No Hit
ATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5830.2540194327044573No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG5810.25314801097991374No Hit
GATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAA5680.24748376977038036No Hit
GGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAA5470.23833384166267266No Hit
ACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5230.2278767809681495No Hit
GGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5040.2195982745849854No Hit
ACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCT4670.20347697268092896RNA PCR Primer, Index 47 (95% over 24bp)
GGTATCAACGCAGAGTACGGGGACCATGCCGGCCAGAACCTGTTGGATCA4610.20086270750729815No Hit
GAGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCA4300.18735567077687246No Hit
GGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4230.1843056947409699No Hit
ACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4000.17428434490871858No Hit
TATCAACGCAGAGTACGGGGACCATGCCGGCCAGAACCTGTTGGATCAAC3960.17254150145963137No Hit
GCGCCGCGAGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCAT3840.16731297111236984No Hit
GGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATCTGTCTCTTATA3770.16426299507646724No Hit
GTATCAACGCAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3670.1599058864537493No Hit
GTATCAACGCAGAGTACGGGAACCCCACAAAAAAAAAAAAAAAAAAAAAA3650.1590344647292057No Hit
TATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3580.1559844886933031No Hit
ATGCCGGCCAGAACCTGTTGGATCAACAACCCCAATCCCTGTCTCTTATA3520.15337022351967236No Hit
GGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCGTACTC3440.14988453662149798No Hit
GATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAA3340.14552742799878002No Hit
GCGAGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCC3260.14204174110060563No Hit
GTGGTATCAACGCAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTT3240.14117031937606203No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG3160.13768463247788768No Hit
TATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2910.12679186092109276No Hit
AGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCCG2860.12461330660973378No Hit
GATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCCGTACTC2740.11938477626247224No Hit
TGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAA2730.11894906540020043No Hit
GGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAA2660.11589908936429785No Hit
TTTTTGGGACCATGCCGGCCAGAACCTGTTGGATCAACAACCCCAATCCA2590.11284911332839528No Hit
GGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCCGTACTCTGCGT2450.10674916125659013No Hit
GGTATCAACGCAGAGTACGGGACCATGCCGGCCAGAACCTGTTGGATCAA2410.10500631780750296No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCC2390.10413489608295935No Hit
GAGTACGGGGACCATGCCGGCCAGAACCTGTTGGATCAACAACCCCAATC2300.10021349832251318No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATCAA1500.076.512561
AAGTACG850.070.011481
ATACGGG700.066.796681
ATTCATT200.00321810163.7604641
ATATAAG1300.062.1255761
GTCAGAT700.060.724252
AGACGCA356.5875774E-660.724252
AAGACGT401.4531528E-553.1337175
ATTCTGG651.7844286E-952.3162731
CTCTTAG250.007783300251.0083661
ATTCAAG250.007783300251.0083661
CCATATT250.007783300251.0083661
TCATGCC250.007783300251.0083663
CGTTAAC250.007790009550.9972539
GTGGTAT10350.050.5155331
TGGTATC10700.050.0549432
TTCAAGA354.814965E-448.57942
GCCTGCA354.814965E-448.57947
TATTAAC354.814965E-448.57944
AGATGTG901.2732926E-1147.229975
Produced by FastQC (version 0.11.5)