FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412179

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412179
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences647060
Sequences flagged as poor quality0
Sequence length61
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG29610.45760825889407475No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGT150.00578903654.9842347
CATTGCG257.8560464E-443.9975929
GTGGTAT8900.039.5759281
TACATCG358.658426E-539.28963541
TGGTATC9050.038.9109422
GCGTCGC300.001923328536.6731640
AGGTCGT300.001925519936.66465822
TTGACGT401.9003777E-434.37577446
CGACGGT350.004099421731.42684610
ACCGCGC350.004099421731.42684618
CTAGTAC553.4879733E-530.0006753
GTATCAA37250.027.8500651
GTCAGCG400.00786976727.5006243
TAGGTCG400.00787273627.49849321
CCGTGCG400.00787273627.49849318
TCGACGG400.00787273627.4984939
GTACTAG550.001221400825.0160331
CCTAGTA550.001223054425.010232
ACCGATC550.001224709625.00442935
GTTAGCC550.001225814225.0005633
Produced by FastQC (version 0.11.5)